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Western blot analysis in Hela cell line lysates (35ug/lane).
Western blot analysis of PAF1 using rabbit polyclonal PAF1 Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the PAF1 gene.
Western blot analysis in Hela cell line lysates (35ug/lane).

PAF1 Antibody

CATALOG NUMBER: 58-886

Clonality:
Polyclonal
Tested Applications:
WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Bovine, Mouse, Rat
Immunogen:
This PAF1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 439-467 amino acids from the C-terminal region of human PAF1.
Conjugate:
Unconjugated
Tested Applications:
WB
Application Note:
For WB starting dilution is: 1:1000
Predicted Molecular Weight:
60 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
PAF1
Additional Names:
RNA polymerase II-associated factor 1 homolog, hPAF1, Pancreatic differentiation protein 2, PAF1, PD2
Protein Accession Number:
Q8N7H5
Protein Gi Number:
182670295
Ncbi Gene Id Number:
54623
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
PAF1, parafibromin (CDC73; MIM 607393), LEO1 (MIM 610507),and CTR9 (MIM 609366) form the PAF protein complex that associateswith the RNA polymerase II subunit POLR2A (MIM 180660) and with ahistone methyltransferase complex (Rozenblatt-Rosen et al., 2005[PubMed 15632063]). The PAF complex also has a role in histonemonoubiquitination (Zhu et al., 2005 [PubMed 16307923]).[suppliedby OMIM].
Background Reference 1:
Muntean, A.G., et al. Cancer Cell 17(6):609-621(2010)
Background Reference 2:
Kim, J., et al. Cell 140(4):491-503(2010)
Background Reference 3:
Moniaux, N., et al. PLoS ONE(9), E7077 (2009) :
Background Reference 4:
Olsen, J.V., et al. Cell 127(3):635-648(2006)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

58-886

List Size:
400 ul

List Price:

$495.00

Datasheet
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