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42-653 (0.3ug/ml) staining of Human Liver lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
42-653 (0.1ug/ml) staining of Mouse (A) and Rat (B) Liver lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
42-653 (3.75ug/ml) staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
42-653 (0.3ug/ml) staining of Human Liver lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.

PAH Antibody

CATALOG NUMBER: 42-653

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC, WB
Host Species:
Goat
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human, Mouse, Rat
Homology:
Expected Species Reactivity based on sequence homology: Dog, Cow
Immunogen:
The immunogen for this antibody is: C-ESRPSRLKKDE
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 48kDa band observed in Human Liver lysate and approx. 52kDa in Mouse and Rat Liver lysates (calculated MW of 51.9kDa according to NP_000268.1). Recommended concentration: 0.1-0.3ug/ml. Primary incubation was 1 hourImmunohistochemistry:Paraffin embedded Human Kidney. Recommended concentration: 3.75ug/ml.
Positive Control 1:
Cat. No. 1304 - Human Liver Tissue Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
PAH
Additional Names:
PAH, phenylalanine hydroxylase, PH, PKU, PKU1
Protein Accession Number:
NP_000268.1
Protein Gi Number:
4557819
Ncbi Gene Id Number:
5053

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

42-653

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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