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46-127 (0.5ug/ml) staining of Human Brain (Cerebral Cortex) lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
46-127 (0.5ug/ml) staining of Human Brain (Cerebral Cortex) lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

PAK1 Antibody

CATALOG NUMBER: 46-127

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Goat
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human, Mouse, Rat
Homology:
Expected Species Reactivity based on sequence homology: Dog
Immunogen:
The immunogen for this antibody is: C-NTEKQKKKPKMSDE
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:1000.Western Blot:Approx 65kDa band observed in Human Brain (Cerebral Cortex and Hippocampus) lysates (calculated MW of 60.6kDa according to NP_002567.3). Recommended concentration: 0.5-1.5ug/ml.
Specificity:
No cross-reactivity exptected with PAK3
Positive Control 1:
Cat. No. 1463 - Rat Brain Tissue Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
PAK1
Additional Names:
PAK1, p21/Cdc42/Rac1-activated kinase 1 (STE20 homolog, yeast) , MGC130000, MGC130001, PAKalpha , p21-activated kinase 1, p21/Cdc42/Rac1-activated kinase 1 (yeast Ste20-related)
Protein Accession Number:
NP_002567.3
Protein Gi Number:
42794769
Ncbi Gene Id Number:
5058
Background Reference 1:
Zhao L, Ma QL, Calon F, Harris-White ME, Yang F, Lim GP, Morihara T, Ubeda OJ, Ambegaokar S, Hansen JE, Weisbart RH, Teter B, Frautschy SA, Cole GM. Role of p21-activated kinase pathway defects in the cognitive deficits of Alzheimer disease. Nat Neurosci. 2006 Feb;9(2):234-42. Epub 2006 Jan 15.

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

46-127

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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