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Western blot analysis of PAK1 using rabbit polyclonal PAK1 Antibody (T423).293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PAK1 gene (Lane 2).
Confocal immunofluorescent analysis of PAK1 Antibody (T423) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).
Formalin-fixed and paraffin-embedded human colon carcinoma tissue reacted with PAK1 Antibody (T423) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Western blot analysis of PAK1 using rabbit polyclonal PAK1 Antibody (T423).293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PAK1 gene (Lane 2).

PAK1 Antibody

CATALOG NUMBER: 63-291

Clonality:
Polyclonal
Tested Applications:
IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Bovine, Mouse, Rat, Rabbit, Xenopus
Immunogen:
This PAK1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 401-430 amino acids from human PAK1.
Conjugate:
Unconjugated
Tested Applications:
IF, IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IF starting dilution is: 1:10~50
For IHC-P starting dilution is: 1:10~50
Predicted Molecular Weight:
61 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
PAK1
Additional Names:
Serine/threonine-protein kinase PAK 1, Alpha-PAK, p21-activated kinase 1, PAK-1, p65-PAK, PAK1
Protein Accession Number:
Q13153
Protein Gi Number:
90111767
Ncbi Gene Id Number:
5058
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
PAK1, a member of the STE20 subfamily of Ser/Thr protein kinases, acts on a variety of targets. It is likely to be the GTPase effector that links the Rho-related GTPases to the JNK MAP kinase pathway. Activity is inhibited in cells undergoing apoptosis, potentially due to binding of CDC2L1 and CDC2L2. The protein interacts tightly with GTP-bound but not GDP-bound CDC42/P21 and RAC1. PAK1 binds to the caspase-cleaved p110 isoform of CDC2L1 and CDC2L2, p110C, but not the full-length proteins. It is a component of cytoplasmic complexes, which also contain PXN, ARHGEF6 and GIT1. The protein is autophosphorylated when activated by CDC42/p21. Structurally, the PAK1 contains 1 CRIB domain.
Background Reference 1:
Chen, S., et al., J. Biol. Chem. 278(22):20029-20036 (2003).
Background Reference 2:
Sells, M.A., et al., Curr. Biol. 7(3):202-210 (1997).
Background Reference 3:
Brown, J.L., et al., Curr. Biol. 6(5):598-605 (1996).

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

63-291

List Size:
400 ul

List Price:

$495.00

Datasheet
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