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Western blot analysis of extracts from LOVO cells treated with starved using PAK1/2 (Phospho-Ser199) Antibody. The lane on the right is treated with the antigen-specific peptide.
Immunohistochemical analysis of paraffin-embedded human placenta tissue using PAK1/2 (Phospho-Ser199) antibody (left) or the same antibody preincubated with blocking peptide (right).
Western blot analysis of extracts from LOVO cells treated with starved using PAK1/2 (Phospho-Ser199) Antibody. The lane on the right is treated with the antigen-specific peptide.

PAK1 (phospho Ser199) Antibody

CATALOG NUMBER: 79-818

Clonality:
Polyclonal
Tested Applications:
IHC, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
PAK1 (phospho Ser199) antibody was raised against a peptide sequence around phosphorylation site of Serine 199 (T-K-S (p)-V-I) derived from Human PAK1.
Conjugate:
Unconjugated
Tested Applications:
IHC, WB
Application Note:
Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100
Specificity:
The antibody detects endogenous levels of PAK1/2 only when phosphorylated at serine 199.
Predicted Molecular Weight:
61 kDa
Purification:
Antibodies were purified by affinity-chromatography using epitope-specific peptide.
Clonality:
Polyclonal
Isotype:
IgG
Physical State:
Liquid
Buffer:
Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration:
1 mg/mL
Storage Conditions:
Store antibody at -20°C for up to one year.
Ncbi Official Symbol:
PAK1
Additional Names:
ADRB2, PAK 1, P65-PAK, P68-PAK
Protein Accession Number:
NP_002567.3
Protein Gi Number:
42794769
Ncbi Gene Id Number:
5058
Background:
The activated kinase acts on a variety of targets. Likely to be the GTPase effector that links the Rho-related GTPases to the JNK MAP kinase pathway. Activated by CDC42 and RAC1. Involved in dissolution of stress fibers and reorganization of focal complexes. Involved in regulation of microtubule biogenesis through phosphorylation of TBCB. Activity is inhibited in cells undergoing apoptosis, potentially due to binding of CDC2L1 and CDC2L2.
Background Reference 1:
Brown J.L., Curr. Biol. 6:598-605(1996).
Background Reference 2:
Sells M.A., Curr. Biol. 7:202-210(1997).
Background Reference 3:
The MGC Project Team; Genome Res. 14:2121-2127(2004).

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

79-818

List Size:
0.1 mg

List Price:

$376.00

Datasheet
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