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Western blot analysis of PAK6 in Raji lysate with PAK6 antibody at (A) 1, (B) 2, and (C) 4 μg/mL.
Immunocytochemistry of PAK6 in Raji cells with PAK6 antibody at 10 μg/mL.
Immunofluorescence of PAK6 in Raji cells with PAK6 antibody at 10 μg/mL.
Western blot analysis of PAK6 in Raji lysate with PAK6 antibody at (A) 1, (B) 2, and (C) 4 μg/mL.
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Trial Size

PAK6 Antibody

CATALOG NUMBER: 3073

Clonality:
Polyclonal
Tested Applications:
ELISA, ICC, IF, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
PAK6 antibody was raised against a 13 amino acid synthetic peptide from near the center of human PAK6.
The immunogen is located within amino acids 260 - 310 of PAK6.
Conjugate:
Unconjugated
Tested Applications:
ELISA, ICC, IF, WB
Application Note:
PAK6 antibody can be used for the detection of PAK6 by Western blot at 1 - 4 μg/mL. Antibody can also be used for immunocytochemistry starting at 10 μg/mL. For immunofluorescence start at 10 μg/mL.
Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Specificity:
PAK6 antibody is predicted to not cross-react with other PAK family proteins.
Positive Control 1:
Cat. No. 1207 - Raji Cell Lysate
Positive Control 2:
Cat. No. 17-007 - Raji Cell Slide
Predicted Molecular Weight:
Predicted: 75 kDa
Observed: 70 kDa
Purification:
PAK6 Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
Physical State:
Liquid
Buffer:
PAK6 Antibody is supplied in PBS containing ≤0.09% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
PAK6 antibody can be stored at 4°C, stable for up to one year. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
PAK6
Additional Names:
PAK6 Antibody: PAK5, PAK5, PAK-5, PAK-6
Protein Accession Number:
NP_064553
Protein Gi Number:
9910476
Ncbi Gene Id Number:
56924
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
PAK6 Antibody: The p21-activated kinases (PAKs) are serine-threonine kinases that bind to the active forms of Cdc42 and Rac. They are divided into two groups, the first of which include PAK1, 2 and 3, and can be activated by Cdc42/Rac binding. Group 1 PAKs contain an autoinhibitory domain whose activity is regulated by Cdc42/Rac binding. The group 1 PAKs are known to be involved in cellular processes such as gene transcription, apoptosis, and cell morphology and motility. Much less is known about the second group, which includes PAK4, 5 and 6. These proteins are not activated by Cdc42/Rac binding. PAK6 was initially identified as an androgen receptor in a yeast two hybrid screen and was found to be highly expressed in testis and prostate tissues. Later experiments have shown it to be activated by MAP kinase kinase 6 and p38 MAP kinase, suggesting that PAK6 may play a role in the cellular response to stress-related signals.
Background Reference 1:
Jaffer ZM and Chernoff J. p21-activated kinases: three more join the Pak. Int. J. Biochem. Cell Biol. 2002; 34:713-7.
Background Reference 2:
Yang F, Li X, Sharma M, et al. Androgen receptor specifically interacts with a novel p21-activated kinase, PAK6. J. Biol. Chem. 2001; 276:15345-53.
Background Reference 3:
Kaur R, Liu X, Gjoerup O, et al. Activation of p21-activated kinase 6 by MAP kinase kinase 6 and p38 MAP kinase. J. Biol. Chem. 2005; 280:3323-30.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

3073

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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