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Western blot analysis of lysates from A-549 cells, using Palladin Rabbit mAb at 1:400 dilution. | Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. | Lysates/proteins: 25μg per lane. | Blocking buffer: 3% nonfat dry milk in TBST. | Detection: ECL Basic Kit. | Exposure time: 90s.
Western blot analysis of lysates from A-549 cells, using Palladin Rabbit mAb at 1:400 dilution. | Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. | Lysates/proteins: 25μg per lane. | Blocking buffer: 3% nonfat dry milk in TBST. | Detection: ECL Basic Kit. | Exposure time: 90s.

Palladin Rabbit Recombinant Antibody (82-691)

CATALOG NUMBER: 82-691

Clonality:
Monoclonal
Tested Applications:
ELISA, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
WB,1:200 - 1:500, ELISA,Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Positive Control 1:
A549
Predicted Molecular Weight:
151kDa
purification:
Affinity purification
Clonality:
Monoclonal
Isotype:
IgG
Physical State:
Liquid
Buffer:
Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3.
Concentration:
Batch dependent
Storage Conditions:
Store at -20℃. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
PALLD
Additional Names:
MYN; PNCA1; CGI151; SIH002; CGI-151; Palladin
Protein Accession Number:
Q8WX93
Ncbi Gene Id Number:
23022
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This gene encodes a cytoskeletal protein that is required for organizing the actin cytoskeleton. The protein is a component of actin-containing microfilaments, and it is involved in the control of cell shape, adhesion, and contraction. Polymorphisms in this gene are associated with a susceptibility to pancreatic cancer type 1, and also with a risk for myocardial infarction. Alternative splicing results in multiple transcript variants.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
This product is for research use only.

CATALOG NUMBER:

82-691

List Size:
100 uL

List Price:

$477.00

Datasheet
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