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Western blot analysis of extracts of HepG2 cells, using PALLD antibody (19-712).<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.
Western blot analysis of extracts of HepG2 cells, using PALLD antibody (19-712).
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.

PALLD Antibody

CATALOG NUMBER: 19-712

Clonality:
Polyclonal
Tested Applications:
Flow, IF, IHC, IP, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Immunogen:
Recombinant Protein of human PALLD
Conjugate:
Unconjugated
Tested Applications:
Flow, IF, IHC, IP, WB
Application Note:
WB: 1:500 - 1:2000
IHC: 1:50 - 1:200
IF: 1:50 - 1:200
IP: 1:20 - 1:50
Flow: 1:20 - 1:50
Positive Control 1:
HepG2
Predicted Molecular Weight:
Observed: 135kDa
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
Physical State:
Liquid
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
PALLD
Additional Names:
sarcoma antigen NY-SAR-77, palladin, SIH002, PNCA1, KIAA0992, FLJ39139, FLJ38193, FLJ22190, CGI-151, cytoskeletal associated protein, PALLD
Ncbi Gene Id Number:
23022
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This gene encodes a cytoskeletal protein that is required for organizing the actin cytoskeleton. The protein is a component of actin-containing microfilaments, and it is involved in the control of cell shape, adhesion, and contraction. Polymorphisms in this gene are associated with a susceptibility to pancreatic cancer type 1, and also with a risk for myocardial infarction. Alternative splicing results in multiple transcript variants.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

19-712

List Size:
50 uL

List Price:

$335.00

Out of stock

Datasheet
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