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Western blot analysis of various lysates using Pan Phospho-Tyrosine Mouse mAb at 1:1000 dilution incubated overnight at 4℃. Jurkat cells and NIH/3T3 cells were treated with Pervanadate (1 mM) at 37℃ for 30 minutes. | Secondary antibody: HRP-conjugated Goat anti-Mouse IgG (H+L) at 1:10000 dilution. | Lysates/proteins: 30 μg per lane. | Blocking buffer: 3% nonfat dry milk in TBST. | Detection: ECL Basic Kit. | Exposure time: 30 s.
Western blot analysis of lysates from C6 cells using Pan Phospho-Tyrosine Mouse mAb at 1:1000 dilution incubated at room temperature for 1.5 hours. C6 cells were treated with pervanadate (1 μM) at 37℃ for 30 minutes. | Secondary antibody: HRP-conjugated Goat anti-Mouse IgG (H+L) at 1:10000 dilution. | Lysates/proteins: 30 μg per lane. | Blocking buffer: 3% nonfat dry milk in TBST. | Detection: ECL Basic Kit. | Exposure time: 10 s.
Immunofluorescence analysis of NIH-3T3 cells using Pan Phospho-Tyrosine Mouse mAb at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Western blot analysis of various lysates using Pan Phospho-Tyrosine Mouse mAb at 1:1000 dilution incubated overnight at 4℃. Jurkat cells and NIH/3T3 cells were treated with Pervanadate (1 mM) at 37℃ for 30 minutes. | Secondary antibody: HRP-conjugated Goat anti-Mouse IgG (H+L) at 1:10000 dilution. | Lysates/proteins: 30 μg per lane. | Blocking buffer: 3% nonfat dry milk in TBST. | Detection: ECL Basic Kit. | Exposure time: 30 s.

Pan Phospho-Tyrosine Mouse Recombinant Antibody (84-555)

CATALOG NUMBER: 84-555

Clonality:
Monoclonal
Tested Applications:
ELISA, IF/ICC, WB
Host Species:
Mouse
Species Reactivity:
Human, Mouse, Other (Wide Range Predicted), Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Mouse
Species Reactivity:
Human, Mouse, Other (Wide Range Predicted), Rat
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF/ICC, WB
Application Note:
WB,1:500 - 1:2000, IF/ICC,1:50 - 1:200, ELISA,Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
purification:
Affinity purification
Clonality:
Monoclonal
Isotype:
IgG2b,Kappa
Physical State:
Liquid
Buffer:
Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide (as specified on the Certificate of Analysis), pH 7.3.
Concentration:
Batch dependent
Storage Conditions:
Store at -20℃. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
pan p-Y
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Protein phosphorylation is one of the main key regulatory mechanisms by which extracellular signals are conveyed. Tyrosine kinase activation is impactful in many tumor types, and in fact, tyrosine kinase inhibitors are one of the more useful therapeutics currently in cancer treatment.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
This product is for research use only.

CATALOG NUMBER:

84-555

List Size:
100 uL

List Price:

$540.00

Datasheet
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