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Western blot analysis of various lysates, using Pan Phospho-Tyrosine Rabbit mAb at 1:2000 dilution. Jurkat cells were treated with Pervanadate (1 mM) at 37℃ for 30 minutes. | Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. | Lysates/proteins: 25μg per lane. | Blocking buffer: 3% nonfat dry milk in TBST. | Detection: ECL Basic Kit. | Exposure time: 90s.
Western blot analysis of lysates from NIH/3T3 cells, using Pan Phospho-Tyrosine Rabbit mAb at 1:2000 dilution. NIH/3T3 cells were treated with Pervanadate (1 mM) at 37℃ for 30 minutes. | Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. | Lysates/proteins: 25μg per lane. | Blocking buffer: 3% nonfat dry milk in TBST. | Detection: ECL Basic Kit. | Exposure time: 90s.
Immunoprecipitation analysis of 300 μg extracts of Jurkat cells treated with Pervanadate using 3 μg Pan Phospho-Tyrosine antibody. Western blot was performed from the immunoprecipitate using Pan Phospho-Tyrosine antibody at a dilution of 1:5000.
Western blot analysis of various lysates, using Pan Phospho-Tyrosine Rabbit mAb at 1:2000 dilution. Jurkat cells were treated with Pervanadate (1 mM) at 37℃ for 30 minutes. | Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. | Lysates/proteins: 25μg per lane. | Blocking buffer: 3% nonfat dry milk in TBST. | Detection: ECL Basic Kit. | Exposure time: 90s.

Pan Phospho-Tyrosine Rabbit Recombinant Antibody (84-641)

CATALOG NUMBER: 84-641

Clonality:
Monoclonal
Tested Applications:
ELISA, IP, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Other (Wide Range Predicted), Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Other (Wide Range Predicted), Rat
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IP, WB
Application Note:
WB,1:1000 - 1:5000, IP,0.5μg-4μg antibody for 200μg-400μg extracts of whole cells, ELISA,Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Predicted Molecular Weight:
>10KDa
purification:
Affinity purification
Clonality:
Monoclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide (as specified on the Certificate of Analysis), pH 7.3.
Concentration:
Batch dependent
Storage Conditions:
Store at -20℃. Avoid freeze / thaw cycles.
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Tyrosine phosphorylation (pTyr), much of which occurred on localized multiple sites, initiates cellular signaling, governs cellular functions, and its dysregulation is implicated in many diseases, especially cancers. pTyr-specific sensing is of great significance for understanding disease states and developing targeted anticancer drugs.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
This product is for research use only.

CATALOG NUMBER:

84-641

List Size:
100 uL

List Price:

$540.00

Datasheet
Shipping Info

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