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Western blot analysis of PAN3 Antibody in Jurkat cell line lysates (35ug/lane)
PAN3 Antibody IHC analysis in formalin fixed and paraffin embedded human cervix carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.
Flow cytometric analysis of Jurkat cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western blot analysis of PAN3 Antibody in Jurkat cell line lysates (35ug/lane)

PAN3 Antibody

CATALOG NUMBER: 61-731

Clonality:
Polyclonal
Tested Applications:
Flow, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Mouse
Immunogen:
This PAN3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 771-799 amino acids from the C-terminal region of human PAN3.
Conjugate:
Unconjugated
Tested Applications:
Flow, IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:50~100
For FACS starting dilution is: 1:10~50
Predicted Molecular Weight:
96 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
PAN3
Additional Names:
PAB-dependent poly(A)-specific ribonuclease subunit PAN3 {ECO:0000255, HAMAP-Rule:MF_03181}, PAB1P-dependent poly(A)-nuclease {ECO:0000255, HAMAP-Rule:MF_03181}, PAN deadenylation complex subunit 3 {ECO:0000255, HAMAP-Rule:MF_03181}, hPan3, PAN3 {ECO:0000255, HAMAP-Rule:MF_03181}
Protein Accession Number:
Q58A45
Protein Gi Number:
341942213
Ncbi Gene Id Number:
255967
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
PAN3 is functions in cytoplasmic mRNA decay. It is as part of the Pan nuclease complex, recruits polyadenylate-binding protein which in turn stimulates PAN2 nuclease activity.
Background Reference 1:
Uchida, N., et al. J. Biol. Chem. 279(2):1383-1391(2004)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

61-731

List Size:
400 ul

List Price:

$495.00

Datasheet
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