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Western blot analysis in NCI-H292 cell line lysates (35ug/lane).
Western blot analysis in NCI-H292 cell line lysates (35ug/lane).

PAPOLG Antibody

CATALOG NUMBER: 56-905

Clonality:
Polyclonal
Tested Applications:
WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This PAPOLG antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 685-714 amino acids from the C-terminal region of human PAPOLG.
Conjugate:
Unconjugated
Tested Applications:
WB
Application Note:
For WB starting dilution is: 1:1000
Predicted Molecular Weight:
83 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
PAPOLG
Additional Names:
Poly(A) polymerase gamma, PAP-gamma, Neo-poly(A) polymerase, Neo-PAP, Polynucleotide adenylyltransferase gamma, SRP RNA 3'-adenylating enzyme, PAPOLG, PAP2, PAPG
Protein Accession Number:
Q9BWT3
Protein Gi Number:
116242699
Ncbi Gene Id Number:
64895
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This gene encodes a member of the poly(A) polymerasefamily which catalyzes template-independent extension of the 3' endof a DNA/RNA strand. This enzyme shares 60% identity to the wellcharacterized poly(A) polymerase II (PAPII) at the amino acidlevel. These two enzymes have similar organization of structuraland functional domains. This enzyme is exclusively localized in thenucleus and exhibits both nonspecific and CPSF (cleavage andpolyadenylation specificity factor)/AAUAAA-dependentpolyadenylation activity. This gene is located on chromosome 2 incontrast to the PAPII gene, which is located on chromosome 14.
Background Reference 1:
Lee, Y.S., et al. J. Biol. Chem. 285(36):28105-28116(2010)
Background Reference 2:
Beausoleil, S.A., et al. Proc. Natl. Acad. Sci. U.S.A. 101(33):12130-12135(2004)
Background Reference 3:
Kyriakopoulou, C.B., et al. J. Biol. Chem. 276(36):33504-33511(2001)
Background Reference 4:
Topalian, S.L., et al. Mol. Cell. Biol. 21(16):5614-5623(2001)

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

56-905

List Size:
400 ul

List Price:

$495.00

Datasheet
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