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46-132 (0.001ug/ml) staining of HeLa (A) and Jurkat (B) lysates (35ug protein in RIPA buffer). Detected by chemiluminescence.
46-132 (0.001ug/ml) staining of Human Cerebellum (A), Human Frontal Cortex (B), Human Hippocampus (C) Mouse Fetal Brain (D), Mouse Brain (E) and Rat Brain (F) lysates (35ug protein in RIPA buffer). Detected by chemiluminescence.
Immunofluorescence staining of MCF7 (A) and Hela (B) cells with 5ug/ml 46-132 antibody. Detected with Rabbit anti-goat IgG-Alexafluor488 antibody at 1:1000. Nuclei Counterstained with DAPI.
46-132 (3.75ug/ml) staining of paraffin embedded Human Thyroid. Steamed antigen retrieval with citrate buffer pH 6, AP-staining. <strong>This data is from a previous batch, not on sale.</strong>
46-132 (3.75ug/ml) staining of paraffin embedded Human Prostate. Steamed antigen retrieval with citrate buffer pH 6, AP-staining. <strong>This data is from a previous batch, not on sale.</strong>
46-132 (0.001ug/ml) staining of HeLa (A) and Jurkat (B) lysates (35ug protein in RIPA buffer). Detected by chemiluminescence.

PARK7 Antibody

CATALOG NUMBER: 46-132

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, WB
Host Species:
Goat
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human, Mouse, Rat
Immunogen:
The immunogen for this antibody is: C-AAQVKAPLVLKD
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 24kDa band observed in lysates of cell lines HeLa and Jurkat and of Human Brain (Cerebellum, Hippocampus, Frontal Cortex) and rodent Brain lysates (calculated MW of 19.9kDa according to NP_001116849.1 and of NP_009193.2). Recommended concentration: 0.05-0.1ug/ml. Primary incubation was 1 hour.Immunofluorescence: Strong expression of the protein seen around the nucleus. Dividing cells appear to show enhanced expression. Recommended concentration: 5ug/ml.
Specificity:
Reported variants (NP_001116849.1; NP_009193.2) represent the same protein.
Positive Control 1:
Cat. No. 1205 - Jurkat Cell Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
PARK7
Additional Names:
PARK7, DJ1, DJ-1, Parkinson disease (autosomal recessive, early onset) 7, oncogene DJ1, CTA-215D11.1, FLJ27376, FLJ34360, FLJ92274, OTTHUMP00000001350, OTTHUMP00000001351, Parkinson disease protein 7, protein DJ-1
Protein Accession Number:
NP_009193.2
Protein Gi Number:
31543380
Ncbi Gene Id Number:
11315
Background Reference 1:
Nagakubo D, Taira T, Kitaura H, Ikeda M, Tamai K, Iguchi-Ariga SM, Ariga H. DJ-1, a novel oncogene which transforms mouse NIH3T3 cells in cooperation with ras. Biochem Biophys Res Commun. 1997 Feb 13;231(2):509-13.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

46-132

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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