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Western blot analysis of PARL in 3T3 cell lysate with PARL antibody at (A) 1 and (B) 2 μg/mL.
Immunocytochemistry of PARL in 3T3 cells with PARL antibody at 2.5 μg/mL.
Immunofluorescence of PARL in 3T3 cells with PARL antibody at 2.50 μg/mL.
Western blot analysis of PARL in 3T3 cell lysate with PARL antibody at (A) 1 and (B) 2 μg/mL.
Trial Size

PARL Antibody

CATALOG NUMBER: 4761

Clonality:
Polyclonal
Tested Applications:
ELISA, ICC, IF, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Homology:
Predicted species reactivity based on immunogen sequence: Bovine: (100%)
Immunogen:
PARL antibody was raised against a 15 amino acid synthetic peptide from near the amino terminus of human PARL.
The immunogen is located within amino acids 40 - 90 of PARL.
Conjugate:
Unconjugated
Tested Applications:
ELISA, ICC, IF, WB
Application Note:
PARL antibody can be used for detection of PARL by Western blot at 1 - 2 μg/mL. Despite its predicted molecular weight of ~42kDa, PARL is observed at a higher molecular weight in SDS-PAGE. Antibody can also be used for immunocytochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in mouse samples; Immunocytochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.
Positive Control 1:
Cat. No. 1282 - 3T3 (NIH) Cell Lysate
Positive Control 2:
Cat. No. 17-201 - 3T3/BALB Cell Slide
Purification:
PARL Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
Physical State:
Liquid
Buffer:
PARL Antibody is supplied in PBS containing ≤0.09% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
PARL antibody can be stored at 4°C, stable for up to one year. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
PARL
Additional Names:
PARL Antibody: PSARL, PSARL1, RHBDS1, PRO2207, PSENIP2, PSARL, Presenilins-associated rhomboid-like protein, mitochondrial, Mitochondrial intramembrane cleaving protease PARL, Pbeta
Protein Accession Number:
Q9H300
Protein Gi Number:
143811433
Ncbi Gene Id Number:
55486
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
PARL Antibody: PARL is a mitochondrial integral membrane protein and a member of the highly conserved rhomboid superfamily of membrane proteins. PARL is required for the control of apoptosis during postnatal growth and is required for the processing of OPA1, a protein that prevents the release of cytochrome C from the mitochondria in response to apoptotic signals. In lymphocytes and neurons, PARL's association with other proteins, such as the Bcl-2 family-related protein Hax1 and Omi can lead to the proteolytic processing of Omi by PARL, preventing the accumulation of Bax, thereby suppressing apoptosis. Variations in this gene have been associated with increased risk for type 2 diabetes. PARL may also play a role in the progression of Alzheimer's disease through its associations with presenilin-1 and -2. Multiple isoforms of PARL are known to exist.
Background Reference 1:
Pellegrini L, Passer BJ, Canelles M, et al. PAMP and PARL, two novel putative metalloproteases interacting with the COOH-terminus of Presenilin-1 and -2. J. Alzheimers Dis.2001; 3:181-90.
Background Reference 2:
Cipolat S, Rudka T, Hartmann D, et al. Mitochondrial rhomboid PARL regulates cytochrome c release during apoptosis via OPA1-dependent cristae remodeling. Cell2006; 126:163-75.
Background Reference 3:
Chao JR, Parganas E, Boyd K, et al. Hax1-mediated processing of HtrA2 by PARL allows survival of lymphocytes and neurons. Nature2008; 452:98-102.
Background Reference 4:
Walder K, Kerr-Bayles L, Civitarese A, et al. The mitochondrial rhomboid protease PSARL is a new candidate gene for type 2 diabetes. Diabetologia2005; 48:459-68.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

4761

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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