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Western blot analysis of extracts of various cell lines, using PARP1 antibody (13-327) at 1:500 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 5s.
Western blot analysis of extracts of Jurkat cells, using PARP1 antibody (13-327) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 5s.
Western blot analysis of extracts from normal (control) and PARP1 knockout (KO) HeLa cells, using PARP1 antibody (13-327) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
Immunohistochemistry of paraffin-embedded human appendix using PARP1 antibody (13-327) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded rat heart using PARP1 antibody (13-327) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded rat spleen using PARP1 antibody (13-327) at dilution of 1:200 (40x lens).
Western blot analysis of extracts of various cell lines, using PARP1 antibody (13-327) at 1:500 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 5s.
KO

PARP1 Antibody, KO Validated

CATALOG NUMBER: 13-327

Clonality:
Polyclonal
Tested Applications:
ChIP, IF, IHC, IP, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 81-390 of human PARP1 (NP_001609.2).
Conjugate:
Unconjugated
Tested Applications:
ChIP, IF, IHC, IP, WB
Application Note:
WB: 1:1000 - 1:3000
IHC: 1:50 - 1:200
IF: 1:50 - 1:200
IP: 1:50 - 1:200
CHIP: 1:50 - 1:200
Positive Control 1:
Jurkat
Positive Control 2:
HeLa
Positive Control 3:
RAW264.7
Positive Control 4:
C6
Positive Control 5:
Mouse testis
Positive Control 6:
Mouse brain
Predicted Molecular Weight:
Observed: 100kDa, 110kDa, 125kDa
Validation:
Antibody is Knockout validated.
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
PARP1
Additional Names:
KIF3B, PARP, PPOL, ADPRT, ARTD1, ADPRT1, PARP-1, ADPRT 1, pADPRT-1
Ncbi Gene Id Number:
142
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This gene encodes a chromatin-associated enzyme, poly(ADP-ribosyl)transferase, which modifies various nuclear proteins by poly(ADP-ribosyl)ation. The modification is dependent on DNA and is involved in the regulation of various important cellular processes such as differentiation, proliferation, and tumor transformation and also in the regulation of the molecular events involved in the recovery of cell from DNA damage. In addition, this enzyme may be the site of mutation in Fanconi anemia, and may participate in the pathophysiology of type I diabetes.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

13-327

List Size:
100 uL

List Price:

$436.00

Datasheet
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