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46-134 (1ug/ml) staining of Human Spleen Lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
46-134 (5ug/ml) staining of paraffin embedded Human Skin. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
46-134 (5ug/ml) staining of paraffin embedded Human Adrenal Gland. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
46-134 (1ug/ml) staining of Human Spleen Lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

PARP2 Antibody

CATALOG NUMBER: 46-134

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC, WB
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Immunogen:
The immunogen for this antibody is: C-LDLFEVEKDGEKE
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:8000.Western Blot:Approx 70kDa band observed in Human Spleen lysates (calculated MW of 60.8kDa according to NP_005475.1. Recommended concentration: 1-3ug/ml.Immunohistochemistry:Paraffin embedded Human Skin and Adrenal Gland. Recommended concentration: 5ug/ml.
Positive Control 1:
Cat. No. 1306 - Human Spleen Tissue Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
PARP2
Additional Names:
PARP2, poly (ADP-ribose) polymerase family, member 2, HGNC:272, ADPRT2, ADPRTL2, ADPRTL3, PARP-2, pADPRT-2 , ADP-ribosyltransferase (NAD poly ADP-ribose polymerase)-like 2, poly (ADP-ribosyl) transferase-like 2, poly(ADP-ribose) synthetase
Protein Accession Number:
NP_005475.1
Protein Gi Number:
4885053
Ncbi Gene Id Number:
10038
Background Reference 1:
Kofler J, Otsuka T, Zhang Z, Noppens R, Grafe MR, Koh DW, Dawson VL, de Murcia JM, Hurn PD, Traystman RJ. Differential effect of PARP-2 deletion on brain injury after focal and global cerebral ischemia. J Cereb Blood Flow Metab. 2005 Jun 15;

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

46-134

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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