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Western blot analysis in mouse bladder tissue lysates (35ug/lane).
Western blot analysis of PARS2 using rabbit polyclonal PARS2 Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the PARS2 gene.
PARS2 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
Flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western blot analysis in mouse bladder tissue lysates (35ug/lane).

PARS2 Antibody

CATALOG NUMBER: 55-619

Clonality:
Polyclonal
Tested Applications:
Flow, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Immunogen:
This PARS2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 390-417 amino acids from the C-terminal region of human PARS2.
Conjugate:
Unconjugated
Tested Applications:
Flow, IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:50~100
For FACS starting dilution is: 1:10~50
Predicted Molecular Weight:
53 kDa
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
PARS2
Additional Names:
Probable proline--tRNA ligase, mitochondrial, Prolyl-tRNA synthetase, ProRS, PARS2
Protein Accession Number:
Q7L3T8
Protein Gi Number:
73919761
Ncbi Gene Id Number:
25973
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Forms part of a macromolecular complex that catalyzes the attachment of specific amino acids to cognate tRNAs during protein synthesis. Modulates the secretion of AIMP1 and may be involved in generation of the inflammatory cytokine EMAP2 from AIMP1.
Background Reference 1:
Lamesch, P., et al. Genomics 89(3):307-315(2007)
Background Reference 2:
Bonnefond, L., et al. Biochemistry 44(12):4805-4816(2005)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

55-619

List Size:
400 ul

List Price:

$495.00

Datasheet
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