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46-135 (0.3ug/ml) staining of Human Cerebellum lysate (35ug protein in RIPA buffer).r. Detected by chemiluminescence.
46-135 (0.15ug/ml) staining of adenovirus-mediated gene transfer in Rat Heart cells 1) Untransfected, 2) transient transfection with Human PVALB. 3) Untransfected Rat Skeletal Muscle. Detected by chemiluminescence.
46-135 (2.5ug/ml) staining of paraffin embedded Human Cortex. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
46-135 Flow cytometric analysis of paraformaldehyde fixed Kelly cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml). IgG control: Unimmunized goat IgG (black line) fo
46-135 (0.3ug/ml) staining of Human Cerebellum lysate (35ug protein in RIPA buffer).r. Detected by chemiluminescence.

Parvalbumin Antibody

CATALOG NUMBER: 46-135

Clonality:
Polyclonal
Tested Applications:
ELISA, Flow, IHC, WB
Host Species:
Goat
Species Reactivity:
Human, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human, Rat
Immunogen:
The immunogen for this antibody is: C-GVDEFSTLVAES
Conjugate:
Unconjugated
Tested Applications:
ELISA, Flow, IHC, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 14-15kDa band observed in Human (Cerebellum) Brain lysates (calculated MW of 12.1kDa according to NP_002845.1). Primary incubation 1 hour at room temperature. Recommended concentration: 0.1-0.3ug/ml. Approx 10-12kDa band also observed in Rat Skeletal Muscle and in Rat Heart cells transfected with human PVALB, but not in untransfected Rat Heart cells. Data kindly provided by Dr Wang Wang, University of Michigan.Immunohistochemistry:Paraffin embedded Human Brain (Cortex). Recommended concentration: 2.5ug/ml.Flow Cytometry: Flow cytometric analysis of Kelly cells. Recommended concentration: 10ug/ml.
Specificity:
Reported variants represent identical protein: NP_002845.1, NP_001302461.1
Positive Control 1:
Cat. No. 1303 - Human Brain Tissue Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
PVALB
Additional Names:
parvalbumin, HGNC:9704, PVALB, D22S749, parvalbumin alpha, MGC116759
Protein Accession Number:
NP_002845.1
Protein Gi Number:
4506335
Ncbi Gene Id Number:
5816
Background Reference 1:
Hashimoto T, Bergen SE, Nguyen QL, Xu B, Monteggia LM, Pierri JN, Sun Z, Sampson AR, Lewis DA. Relationship of brain-derived neurotrophic factor and its receptor TrkB to altered inhibitory prefrontal circuitry in schizophrenia. J Neurosci. 2005 Jan 12;25(2):372-83.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

46-135

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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