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46-138 (0.3ug/ml) staining of Human Duodenum lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
46-138 (4.5ug/ml) staining of paraffin embedded Human Esophagus. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.
46-138 Negative Control showing staining of paraffin embedded Human Esophagus, with no primary antibody.
46-138 (0.3ug/ml) staining of Human Duodenum lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.

PAX3 Antibody

CATALOG NUMBER: 46-138

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC, WB
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Homology:
Expected Species Reactivity based on sequence homology: Rat, Cow
Immunogen:
The immunogen for this antibody is: TTLAGAVPRMM-C
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 45kDa band observed in lysates of Human Duodenum, Skin, and in lysates of cell lines A431 (calculated MW of 45.2kDa according to NP_852125.1 and of 44.8kDa according to NP_852126.1). Recommended concentration: 0.3-1ug/ml. Primary incubation was 1 hour.Immunohistochemistry:In paraffin embedded Human Esophagus expression of PAX3 is detected in squamous epithelium. Recommended concentration: 4.5ug/ml.
Specificity:
This antibody is expected to recognize all 8 reported isoforms (NP_852122.1; NP_000429.2; NP_039230.1; NP_852123.1; NP_852124.1; NP_852126.1; NP_852125.1; NP_001120838.1).
Positive Control 1:
Cat. No. 1365 - Human Esophagus Tissue Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
PAX3
Additional Names:
PAX3, WS1, CDHS, HUP2, paired box gene 3 (Waardenburg syndrome 1), paired domain gene 3, paired box homeotic gene 3 (Waardenburg syndrome 1), paired box 3, MGC120381, MGC120382, MGC120383, MGC120384, MGC134778, paired box homeotic gene 3, paired domain gene HuP2
Protein Accession Number:
NP_852122.1,NP_000429.2,NP_039230.1,NP_852123.1,NP_852124.1,NP_852126.1,NP_852125.1,NP_001120838.1
Protein Gi Number:
6654638
Ncbi Gene Id Number:
5077
Background Reference 1:
Tsukamoto K, Nakamura Y, Niikawa N. Isolation of two isoforms of the PAX3 gene transcripts and their tissue-specific alternative expression in human adult tissues. Hum Genet. 1994 Mar;93(3):270-4.

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

46-138

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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