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42-499 (0.2ug/ml) staining of Mouse Pancreas lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
42-499 (1.5ug/ml) as the reporter antibody.
42-499 (0.2ug/ml) staining of Mouse Pancreas lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

PAX4 Antibody

CATALOG NUMBER: 42-499

Clonality:
Polyclonal
Tested Applications:
EIA, ELISA, WB
Host Species:
Goat
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human, Mouse
Homology:
Expected Species Reactivity based on sequence homology: Rat, Dog
Immunogen:
The immunogen for this antibody is: C-GKLATATSLPEDTVR
Conjugate:
Unconjugated
Tested Applications:
EIA, ELISA, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:64000.Western Blot:Approx 37kDa band observed in Mouse Pancreas lysates and in lysates of cell lines A431, HeLa and K562 (calculated MW of 37.1kDa according to Human NP_006184.2 and 38.0kDa according to Mouse NP_035168.1). Recommended concentration: 0.2-0.6ug/ml.Enzyme immunoassay: Sandwich-type ELISA with increasing amount of recombinant PAX4 captured by a rabbit antibody. Recommended reporter concentration: 1-2ug/ml
Positive Control 1:
Cat. No. 1414 - Mouse Pancreas Tissue Lysate
Positive Control 2:
Cat. No. 1203 - A549 Cell Lysate
Positive Control 3:
Cat. No. 1204 - K562 Cell Lysate
Positive Control 4:
Cat. No. 1201 - HeLa Cell Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
PAX4
Additional Names:
PAX4, paired box 4, KPD, MGC129960, MODY9, paired box gene 4, paired domain gene 4
Protein Accession Number:
NP_006184.2
Protein Gi Number:
170784824
Ncbi Gene Id Number:
5078

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

42-499

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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