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Western Blot at 1:2000 dilution Lane 1: Hela whole cell lysates Lane 2: rat liver lysates Lysates/proteins at 20 ug per lane.
Western blot analysis of PAX4 antibody in CEM cell line lysates (35ug/lane)
Formalin-fixed and paraffin-embedded human brain tissue reacted with PAX4 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Flow cytometric analysis of widr cells using PAX4 Antibody (bottom histogram) compared to a negative control cell (top histogram)FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western Blot at 1:2000 dilution Lane 1: Hela whole cell lysates Lane 2: rat liver lysates Lysates/proteins at 20 ug per lane.

PAX4 Antibody

CATALOG NUMBER: 62-404

Clonality:
Polyclonal
Tested Applications:
Flow, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Rat
Homology:
Predicted species reactivity based on immunogen sequence: Mouse
Immunogen:
This PAX4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 171-200 amino acids from the Central region of human PAX4.
Conjugate:
Unconjugated
Tested Applications:
Flow, IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:10~50
For FACS starting dilution is: 1:10~50
Predicted Molecular Weight:
38 kDa
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
PAX4
Additional Names:
Paired box protein Pax-4, PAX4
Protein Accession Number:
O43316
Ncbi Gene Id Number:
5078
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
PAX4 is a member of the paired box (PAX) family of transcription factors. These proteins play critical roles during fetal development and cancer growth. The paired box 4 gene is involved in pancreatic islet development and mouse studies have demonstrated a role for this gene in differentiation of insulin-producing beta cells.
Background Reference 1:
Hata,S., Int. J. Oncol. 33 (5), 1065-1071 (2008)
Background Reference 2:
Zhang,Y., Diabetes Res. Clin. Pract. 81 (3), 365-369 (2008)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

62-404

List Size:
400 ul

List Price:

$495.00

Datasheet
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