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Antibody used in IHC on Human kidney.
Antibody used in WB on Human Jurkat 0.2-1 ug/ml.
Antibody used in WB on Human 721_B at 1 ug/ml.
Antibody used in IHC on Human kidney.

PAX5 Antibody

CATALOG NUMBER: 28-164

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC, WB
Host Species:
Rabbit
Species Reactivity:
Dog, Human, Mouse, Rat, Zebrafish
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Dog, Human, Mouse, Rat, Zebrafish
Immunogen:
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human PAX5.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC, WB
Application Note:
PAX5 antibody can be used for detection of PAX5 by ELISA at 1:312500. PAX5 antibody can be used for detection of PAX5 by western blot at 2.0 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Positive Control 1:
Cat. No. 1205 - Jurkat Cell Lysate
Predicted Molecular Weight:
42 kDa
Purification:
Antibody is purified by peptide affinity chromatography method.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Concentration:
batch dependent
Storage Conditions:
For short periods of storage (days) store at 4°C. For longer periods of storage, store PAX5 antibody at -20°C. As with any antibody avoid repeat freeze-thaw cycles.
Ncbi Official Symbol:
PAX5
Additional Names:
PAX5, ALL3, BSAP
Protein Accession Number:
NP_057953
Protein Gi Number:
9951920
Ncbi Gene Id Number:
5079
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The PAX5 gene is a member of the paired box (PAX) family of transcription factors. The central feature of this gene family is a novel, highly conserved DNA-binding motif, known as the paired box. The PAX proteins are important regulators in early development, and alterations in the expression of their genes are thought to contribute to neoplastic transformation. The PAX5 gene encodes the B-cell lineage specific activator protein (BSAP) that is expressed at early, but not late stages of B-cell differentiation. Its expression has also been detected in developing CNS and testis, therefore, PAX5 gene product may not only play an important role in B-cell differentiation, but also in neural development and spermatogenesis.
Background Reference 1:
Robichaud, G.A., et al., (2004) J. Biol. Chem. 279 (48), 49956-49963.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
This product is for research use only.

CATALOG NUMBER:

28-164

List Size:
100 ul

List Price:

$519.00

Datasheet
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