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45-105 (0.3ug/ml) staining of Human Lymph lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
45-105 (1ug/ml) staining of Mouse (A) and (0.3ug/ml) Rat (B) Spleen lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescen
45-105 (0.3ug/ml) staining of Human Lymph lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

PAX5 Antibody

CATALOG NUMBER: 45-105

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Goat
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human, Mouse, Rat
Immunogen:
The immunogen for this antibody is: DLEKNYPTPRTSR-C
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:64000.Western Blot:Approx 38kDa band observed in Human Lymph and Rat Spleen lysates, and approx.40kDa in Mouse Spleen lysates (calculated MW of 42.1kDa according to Human NP_057953.1 and 42.2kDa according to Mouse NP_032808.1 and Rat NP_001102731.1). Recommended concentration: 0.1-0.5ug/ml.
Positive Control 1:
Cat. No. 1215 - U937 Cell Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
PAX5
Additional Names:
PAX5, BSAP, paired box gene 5 (B-cell lineage specific activator protein), B-cell lineage specific activator protein, paired box homeotic gene 5 (B-cell lineage specific activator protein), paired box 5, B cell specific activator protein, B-cell lineage specific activator, paired box homeotic gene 5, transcription factor PAX 5
Protein Accession Number:
NP_057953.1
Protein Gi Number:
9951920
Ncbi Gene Id Number:
5079
Background Reference 1:
Adams B, Dorfler P, Aguzzi A, Kozmik Z, Urbanek P, Maurer-Fogy I, Busslinger M. Pax-5 encodes the transcription factor BSAP and is expressed in B lymphocytes, the developing CNS, and adult testis. Genes Dev 1992 Sep;6(9):1589-607.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

45-105

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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