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46-140 (0.5ug/ml) staining of Mouse Eye lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
46-140 (0.5ug/ml) staining of Mouse Eye lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.

PAX6 Antibody

CATALOG NUMBER: 46-140

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Goat
Species Reactivity:
Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Mouse
Homology:
Expected Species Reactivity based on sequence homology: Human, Rat
Immunogen:
The immunogen for this antibody is: C-REEKLRNQRRQASN
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 50kDa band observed in Mouse Eye lysates (calculated MW of 48.2kDa according to Human NP_001595.2 and to Mouse NP_038655.1). Recommended concentration: 0.3-1ug/ml. Primary incubation was 1 hour.
Specificity:
This antibody is expected to recognise both reported isoforms (NP_000271.1 and NP_001595.2).
Positive Control 1:
Cat. No. 1420 - Mouse Eye Tissue Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
PAX6
Additional Names:
PAX6, paired box gene 6 (aniridia, keratitis), AN, AN2, D11S812E, MGC17209, MGDA, WAGR, paired box gene 6, paired box homeotic gene 6 (aniridia, keratitis), paired box homeotic gene-6
Protein Accession Number:
NP_000271.1,NP_001595.2
Protein Gi Number:
4505615
Ncbi Gene Id Number:
5080
Background Reference 1:
von Holst A, Egbers U, Prochiantz A, Faissner A. Neural stem/progenitor cells express 20 tenascin C isoforms that are differentially regulated by pax6. J Biol Chem. 2007 Feb 12;

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

46-140

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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