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Western blot analysis of PAX6 using rabbit polyclonal PAX6 Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the PAX6 gene.
PAX6 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
Confocal immunofluorescent analysis of PAX6 Antibody with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).DAPI was used to stain the cell nuclear (blue).
Western blot analysis of PAX6 using rabbit polyclonal PAX6 Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the PAX6 gene.

PAX6 Antibody

CATALOG NUMBER: 56-734

Clonality:
Polyclonal
Tested Applications:
IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This PAX6 antibody is generated from rabbits immunized with a recombinant protein from human PAX6.
Conjugate:
Unconjugated
Tested Applications:
IF, IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:10~50
For IF starting dilution is: 1:10~50
Predicted Molecular Weight:
47 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
PAX6
Additional Names:
Paired box protein Pax-6, Aniridia type II protein, Oculorhombin, PAX6, AN2
Protein Accession Number:
P26367
Protein Gi Number:
6174889
Ncbi Gene Id Number:
5080
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This gene encodes paired box gene 6, one of many humanhomologs of the Drosophila melanogaster gene prd. In addition tothe hallmark feature of this gene family, a conserved paired boxdomain, the encoded protein also contains a homeo box domain. Bothdomains are known to bind DNA, and function as regulators of genetranscription. This gene is expressed in the developing nervoussystem, and in developing eyes. Mutations in this gene are known tocause ocular disorders such as aniridia and Peter's anomaly.Alternatively spliced transcript variants encoding either the sameor different isoform have been found for this gene. [provided byRefSeq].
Background Reference 1:
Gosmain, Y., et al. J. Biol. Chem. 285(43):33381-33393(2010)
Background Reference 2:
Vuzman, D., et al. Biophys. J. 99(4):1202-1211(2010)
Background Reference 3:
Zhang, X., et al. Cell Stem Cell 7(1):90-100(2010)
Background Reference 4:
Bremond-Gignac, D., et al. Mol. Vis. 16, 1705-1711 (2010) :

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

56-734

List Size:
400 ul

List Price:

$495.00

Datasheet
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