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46-141 (1ug/ml) staining of Human Kidney (A) and HeLa cell (B) lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
46-141 (5ug/ml) staining of paraffin embedded Human Kidney Cortex. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
46-141 (1ug/ml) staining of Human Kidney (A) and HeLa cell (B) lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.

PAX8 Antibody

CATALOG NUMBER: 46-141

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC, WB
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Rat
Immunogen:
The immunogen for this antibody is: PHNSIRSGHGGLN-C
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 35kDa band observed in Human Kidney lysates. An additional 48kDa was also observed in some Kidney and HeLa cell lysates (calculated MW of 34.9kDa according to Human NP_039247.1 PAX8D and 48.2kDa according to Human NP_003457.1 PAXA). Recommended concentration: 1-2ug/ml. Primary incubation 1 hour at room temperature.Immunohistochemistry:Paraffin embedded Human Kidney Cortex. Recommended concentration: 5ug/ml.
Specificity:
This antibody is expected to recognise all five human isoforms (as represented by NP_003457.1; NP_039245.1; NP_039246.1; NP_039247.1; NP_054698.1)
Positive Control 1:
Cat. No. 1380 - Human Thyroid Tissue Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
PAX8
Additional Names:
PAX8, paired box gene 8, paired domain gene 8, paired box 8
Protein Accession Number:
NP_003457.1,NP_039245.1,NP_039246.1,NP_039247.1,NP_054698.1
Protein Gi Number:
5803117
Ncbi Gene Id Number:
7849
Background Reference 1:
Pasca di Magliano M, Di Lauro R, Zannini M. Pax8 has a key role in thyroid cell differentiation. Proc Natl Acad Sci USA. 2000 Nov 21;97(24):13144-9.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

46-141

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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