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Western blot analysis of lysed extracts from HT29 cells untreated or treated with PMA using Paxillin (Phospho-Tyr31).
Western blot analysis of lysed extracts from HT29 cells untreated or treated with PMA using Paxillin (Phospho-Tyr31).

Paxillin (phospho Tyr31) Antibody

CATALOG NUMBER: 79-470

Clonality:
Polyclonal
Tested Applications:
WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Paxillin (Phospho-Tyr31) antibody was raised against a peptide sequence around phosphorylation site of tyrosine 31 (T-P-Y (p) -S-Y) derived from Human Paxillin.
Conjugate:
Unconjugated
Tested Applications:
WB
Application Note:
Western Blot: 1:500~1:1000
Specificity:
This antibody detects endogenous level of Paxillin only when phosphorylated at tyrosine 31.
Predicted Molecular Weight:
68 kDa
Purification:
Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration:
1 mg/mL
Storage Conditions:
Store antibody at -20°C for up to one year.
Ncbi Official Symbol:
PXN
Additional Names:
NACP, PARK1, PAXI, PXN
Protein Accession Number:
NP_001074324.1
Protein Gi Number:
170932514
Ncbi Gene Id Number:
5829
Background:
An antiphosphotyrosine antibody was used to identify proteins that are phosphorylated in Rous sarcoma virus-transformed chick embryo fibroblasts, and a 76-kD protein was obtained that localizes to focal adhesions at the ends of actin-containing stress fibers in nontransformed cells (Ref.1). This protein was purified from chicken gizzard smooth muscle, and was named Pxn (Paxillin) ('paxillus' means 'small stake' or 'peg' in Latin) as a protein tethered to the membrane at focal adhesions
Background Reference 1:
Davidson D, et al. (2001) EMBO J 20 (13): 3414-3426. Fleming I, et al. (1999) Proc Natl Acad Sci U S A 96 (3): 1123-1128. Goldberg MB. (2001) Microbiol Mol Biol Rev 65 (4): 595-626. Kook S, et al. (2000) Mol Biol Cell 11 (3): 929-939.

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

79-470

List Size:
0.1 mg

List Price:

$376.00

Datasheet
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