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Antibody used in WB on Human Jurkat 2.5 ug/ml.
Antibody used in WB on Human Jurkat 2.5 ug/ml.

PBEF1 Antibody

CATALOG NUMBER: 29-725

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Rabbit
Species Reactivity:
Dog, Human, Mouse, Rat, Zebrafish
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Dog, Human, Mouse, Rat, Zebrafish
Immunogen:
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human PBEF1.
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
PBEF1 antibody can be used for detection of PBEF1 by ELISA at 1:312500. PBEF1 antibody can be used for detection of PBEF1 by western blot at 2.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Positive Control 1:
Cat. No. 1205 - Jurkat Cell Lysate
Predicted Molecular Weight:
54 kDa
Purification:
Antibody is purified by protein A chromatography method.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Concentration:
batch dependent
Storage Conditions:
For short periods of storage (days) store at 4°C. For longer periods of storage, store PBEF1 antibody at -20°C. As with any antibody avoid repeat freeze-thaw cycles.
Ncbi Official Symbol:
NAMPT
Additional Names:
PBEF1, 1110035O14Rik, DKFZP666B131, MGC117256, NAMPT, PBEF, VF, PBEF1, VISFATIN
Protein Accession Number:
NP_005737
Protein Gi Number:
5031977
Ncbi Gene Id Number:
10135
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
PBEF1 catalyzes the condensation of nicotinamide with 5-phosphoribosyl-1-pyrophosphate to yield nicotinamide mononucleotide, one step in the biosynthesis of nicotinamide adenine dinucleotide. The protein is an adipokine that is localized to the bloodstream and has various functions, including the promotion of vascular smooth muscle cell maturation and inhibition of neutrophil apoptosis. It also activates insulin receptor and has insulin-mimetic effects, lowering blood glucose and improving insulin sensitivity. The protein is highly expressed in visceral fat and serum levels of the protein correlate with obesity.
Background Reference 1:
Chen, M.P., (2006) J. Clin. Endocrinol. Metab. 91 (1), 295-299.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
This product is for research use only.

CATALOG NUMBER:

29-725

List Size:
100 ul

List Price:

$473.00

Datasheet
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