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Western Blot at 1:1000 dilution + Hela whole cell lysate Lysates/proteins at 20 ug per lane.
Formalin-fixed and paraffin-embedded human testis tissue reacted wit , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Western Blot at 1:1000 dilution + Hela whole cell lysate Lysates/proteins at 20 ug per lane.

PBK Antibody

CATALOG NUMBER: 62-697

Clonality:
Polyclonal
Tested Applications:
IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Mouse
Immunogen:
This PBK antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 286-317 amino acids from the C-terminal region of human PBK.
Conjugate:
Unconjugated
Tested Applications:
IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:10~50
Predicted Molecular Weight:
36 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
PBK
Additional Names:
Lymphokine-activated killer T-cell-originated protein kinase, Cancer/testis antigen 84, CT84, MAPKK-like protein kinase, Nori-3, PDZ-binding kinase, Spermatogenesis-related protein kinase, SPK, T-LAK cell-originated protein kinase, PBK, TOPK
Protein Accession Number:
Q96KB5
Protein Gi Number:
83305809
Ncbi Gene Id Number:
55872
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
PBK is a serine/threonine kinase related to the dual specific mitogen-activated protein kinase kinase (MAPKK) family. Evidence suggests that mitotic phosphorylation is required for its catalytic activity. This mitotic kinase may be involved in the activation of lymphoid cells and support testicular functions, with a suggested role in the process of spermatogenesis.
Background Reference 1:
Nandi, A., et al., Blood Cells Mol. Dis. 32(1):240-245 (2004).
Background Reference 2:
Simons-Evelyn, M., et al., Blood Cells Mol. Dis. 27(5):825-829 (2001).
Background Reference 3:
Zhao, S., et al., Int. J. Biochem. Cell Biol. 33(6):631-636 (2001).
Background Reference 4:
Abe, Y., et al., J. Biol. Chem. 275(28):21525-21531 (2000).

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

62-697

List Size:
400 ul

List Price:

$495.00

Datasheet
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