Western blot analysis of extracts of various cell lines, using PBRM1 antibody (23-955) at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit._Exposure time: 30s.

PBRM1 Antibody

CATALOG NUMBER: 23-955

Clonality:

Polyclonal

Tested Applications:

Host Species:

Rabbit

Species Reactivity:

Human, Mouse, Rat

Conjugate:

Unconjugated

Specifications

Host Species:

Rabbit

Species Reactivity:

Human, Mouse, Rat

Immunogen:

Recombinant fusion protein containing a sequence corresponding to amino acids 1-230 of human PBRM1 (NP_060783.3).

Conjugate:

Unconjugated

Tested Applications:

Application Note:

WB: 1:500 - 1:2000

Positive Control 1:

HeLa

Positive Control 2:

Jurkat

Positive Control 3:

BT-474

Positive Control 4:

NIH/3T3

Positive Control 5:

LO2

Positive Control 6:

Mouse lung

Predicted Molecular Weight:

Observed: 230kDa

Advanced Validation

Properties

Purification:

Affinity purification

Clonality:

Polyclonal

Isotype:

IgG

Physical State:

Liquid

Buffer:

PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Concentration:

batch dependent

Storage Conditions:

Store at -20°C. Avoid freeze / thaw cycles.

Additional Info

Ncbi Official Symbol:

PBRM1

Additional Names:

PBRM1, BAF180, MGC156155, MGC156156, PB1

Ncbi Gene Id Number:

55193

User Note:

Optimal dilutions for each application to be determined by the researcher.

Background

Background:

This locus encodes a subunit of ATP-dependent chromatin-remodeling complexes. The encoded protein has been identified as in integral component of complexes necessary for ligand-dependent transcriptional activation by nuclear hormone receptors. Mutations at this locus have been associated with primary clear cell renal cell carcinoma.

Citations

Set Contents

Disclaimer

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:

Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

23-955

List Size:

100 uL

List Price:

$436.00

Datasheet

Shipping Info

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$35 Dry Ice Shipment fee may be required on some items.

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PBRM1 Antibody

Clonality:

Tested Applications:

Host Species:

Species Reactivity:

Conjugate:

Host Species:

Species Reactivity:

Immunogen:

Conjugate:

Tested Applications:

Application Note:

Positive Control 1:

Positive Control 2:

Positive Control 3:

Positive Control 4:

Positive Control 5:

Positive Control 6:

Predicted Molecular Weight:

Purification:

Clonality:

Isotype:

Physical State:

Buffer:

Concentration:

Storage Conditions:

Ncbi Official Symbol:

Additional Names:

Ncbi Gene Id Number:

User Note:

Background:

Disclaimer:

List Size:

Shipping Info

Customize your order

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