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Western Blot at 1:1000 dilution + HepG2 whole cell lysate Lysates/proteins at 20 ug per lane.
Western blot analysis in mouse liver tissue lysates (35ug/lane).
PC Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human pancreas tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of PC Antibody for immunohistochemistry.
Western Blot at 1:1000 dilution + HepG2 whole cell lysate Lysates/proteins at 20 ug per lane.

PC Antibody

CATALOG NUMBER: 56-463

Clonality:
Polyclonal
Tested Applications:
IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Homology:
Predicted species reactivity based on immunogen sequence: Bovine, Rat
Immunogen:
This PC antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 53-82 amino acids from the N-terminal region of human PC.
Conjugate:
Unconjugated
Tested Applications:
IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:10~50
Predicted Molecular Weight:
130 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
physical-state:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
PC
Additional Names:
Pyruvate carboxylase, mitochondrial, Pyruvic carboxylase, PCB, PC
Protein Accession Number:
P11498
Protein Gi Number:
1709947
Ncbi Gene Id Number:
5091
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This gene encodes pyruvate carboxylase, which requiresbiotin and ATP to catalyse the carboxylation of pyruvate tooxaloacetate. The active enzyme is a homotetramer arranged in atetrahedron which is located exclusively in the mitochondrialmatrix. Pyruvate carboxylase is involved in gluconeogenesis,lipogenesis, insulin secretion and synthesis of theneurotransmitter glutamate. Mutations in this gene have beenassociated with pyruvate carboxylase deficiency. Alternativelyspliced transcript variants with different 5' UTRs, but encodingthe same protein, have been found for this gene. [provided byRefSeq].
Background Reference 1:
MacDonald, M.J., et al. Diabetologia 52(6):1087-1091(2009)
Background Reference 2:
Monnot, S., et al. Hum. Mutat. 30(5):734-740(2009)
Background Reference 3:
Wang, D., et al. Mol. Genet. Metab. 95 (1-2), 31-38 (2008) :
Background Reference 4:
Vora, S., et al. Natl MedIndia 21(3):116-119(2008)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

56-463

List Size:
400 ul

List Price:

$495.00

Datasheet
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