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Antibody used in WB on Human HepG2 at 2.5 ug/ml.
Antibody used in WB on Human HepG2 at 2.5 ug/ml.

PCAF Antibody

CATALOG NUMBER: 27-607

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Rabbit
Species Reactivity:
Dog, Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Dog, Human, Mouse
Immunogen:
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human PCAF.
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
PCAF antibody can be used for detection of PCAF by ELISA at 1:62500. PCAF antibody can be used for detection of PCAF by western blot at 2.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Positive Control 1:
Cat. No. 1211 - HepG2 Cell Lysate
Predicted Molecular Weight:
93 kDa
Purification:
Antibody is purified by protein A chromatography method.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Concentration:
batch dependent
Storage Conditions:
For short periods of storage (days) store at 4°C. For longer periods of storage, store PCAF antibody at -20°C. As with any antibody avoid repeat freeze-thaw cycles.
Ncbi Official Symbol:
KAT2B
Additional Names:
PCAF, P, CAF, PCAF, P/CAF
Protein Accession Number:
NP_003875
Protein Gi Number:
40805843
Ncbi Gene Id Number:
8850
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
CBP and p300 are large nuclear proteins that bind to many sequence-specific factors involved in cell growth and/or differentiation, including c-jun and the adenoviral oncoprotein E1A. PCAF associates with p300/CBP. It has in vitro and in vivo binding activity with CBP and p300, and competes with E1A for binding sites in p300/CBP. It has histone acetyl transferase activity with core histones and nucleosome core particles, indicating that this protein plays a direct role in transcriptional regulation.CBP and p300 are large nuclear proteins that bind to many sequence-specific factors involved in cell growth and/or differentiation, including c-jun and the adenoviral oncoprotein E1A. The protein encoded by this gene associates with p300/CBP. It has in vitro and in vivo binding activity with CBP and p300, and competes with E1A for binding sites in p300/CBP. It has histone acetyl transferase activity with core histones and nucleosome core particles, indicating that this protein plays a direct role in transcriptional regulation.
Background Reference 1:
Chu, B.Y., Biochem. J. 392 (PT 3), 589-599 (2005).

FOR RESEARCH USE ONLY

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Disclaimer:
This product is for research use only.

CATALOG NUMBER:

27-607

List Size:
100 ul

List Price:

$473.00

Datasheet
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