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Western blot analysis in MDA-MB231 cell line lysates (35ug/lane).
Western blot analysis in MDA-MB231 cell line lysates (35ug/lane).

PCAF Antibody

CATALOG NUMBER: 56-212

Clonality:
Polyclonal
Tested Applications:
WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Mouse
Immunogen:
This PCAF antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 253-283 amino acids from the N-terminal region of human PCAF.
Conjugate:
Unconjugated
Tested Applications:
WB
Application Note:
For WB starting dilution is: 1:1000
Predicted Molecular Weight:
93 kDa
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
KAT2B
Additional Names:
Histone acetyltransferase KAT2B, Histone acetyltransferase PCAF, Histone acetylase PCAF, Lysine acetyltransferase 2B, P300/CBP-associated factor, P/CAF, KAT2B, PCAF
Protein Accession Number:
Q92831
Protein Gi Number:
83287776
Ncbi Gene Id Number:
8850
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
CBP and p300 are large nuclear proteins that bind to manysequence-specific factors involved in cell growth and/ordifferentiation, including c-jun and the adenoviral oncoproteinE1A. The protein encoded by this gene associates with p300/CBP. Ithas in vitro and in vivo binding activity with CBP and p300, andcompetes with E1A for binding sites in p300/CBP. It has histoneacetyl transferase activity with core histones and nucleosome coreparticles, indicating that this protein plays a direct role intranscriptional regulation.
Background Reference 1:
Perez, R.E., et al. J. Cell. Physiol. 225(2):394-405(2010)
Background Reference 2:
Mooney, S.M., et al. J. Biol. Chem. 285(40):30443-30452(2010)
Background Reference 3:
Aoyama, T., et al. J. Biol. Chem. 285(39):29842-29850(2010)
Background Reference 4:
Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010) :

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

56-212

List Size:
400 ul

List Price:

$495.00

Datasheet
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