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42-696 (0.01ug/ml) staining of HeLa lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
42-696 (5ug/ml) staining of paraffin embedded Human Lung. Steamed antigen retrieval with citrate buffer Ph 6, AP-staining.
42-696 (0.01ug/ml) staining of HeLa lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

PCBP1 Antibody

CATALOG NUMBER: 42-696

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC, WB
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Rat, Dog, Pig, Cow
Immunogen:
The immunogen for this antibody is: C-ADLAKLNQVARQQSH
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 38kDa band observed in lysates of cell line HeLa and in Human Placenta lysates (calculated MW of 37.5kDa according to NP_006187.2). Recommended concentration: 0.01-0.03ug/ml.Immunohistochemistry:Paraffin embedded Human Lung (respiratory epithelium). Recommended concentration: 5ug/ml.
Positive Control 1:
Cat. No. 1309 - Human Placenta Lysate
Positive Control 2:
Cat. No. 1201 - HeLa Cell Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
PCBP1
Additional Names:
PCBP1, poly(rC) binding protein 1, HNRPE1, HNRPX, hnRNP-E1, hnRNP-X, alpha-CP1, heterogeneous nuclear ribonucleoprotein E1, heterogenous nuclear ribonucleoprotein E1, heterogenous nuclear ribonucleoprotein X, nucleic acid binding protein sub 2.3
Protein Accession Number:
CAA55016.1
Protein Gi Number:
222352151
Ncbi Gene Id Number:
5093

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

42-696

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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