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Western Blot at 1:2000 dilution Lane 1: Hela whole cell lysate Lane 2: HepG2 whole cell lysate Lane 3: K562 whole cell lysate Lane 4: 293T/17 whole cell lysate Lane 5: mouse skeletal muscle lysate Lane 6: mouse spleen lysate Lysates/proteins at 20 ug per lane.
Western blot analysis in K562 cell line lysates (35ug/lane).
Western Blot at 1:2000 dilution Lane 1: Hela whole cell lysate Lane 2: HepG2 whole cell lysate Lane 3: K562 whole cell lysate Lane 4: 293T/17 whole cell lysate Lane 5: mouse skeletal muscle lysate Lane 6: mouse spleen lysate Lysates/proteins at 20 ug per lane.

PCBP1 Antibody

CATALOG NUMBER: 60-150

Clonality:
Polyclonal
Tested Applications:
WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Homology:
Predicted species reactivity based on immunogen sequence: Bovine, Rabbit
Immunogen:
This PCBP1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 188-217 amino acids from the Central region of human PCBP1.
Conjugate:
Unconjugated
Tested Applications:
WB
Application Note:
For WB starting dilution is: 1:1000
Predicted Molecular Weight:
37 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
PCBP1
Additional Names:
Poly(rC)-binding protein 1, Alpha-CP1, Heterogeneous nuclear ribonucleoprotein E1, hnRNP E1, Nucleic acid-binding protein SUB23, PCBP1
Protein Accession Number:
Q15365
Protein Gi Number:
42560548
Ncbi Gene Id Number:
5093
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This intronless gene is thought to have been generated byretrotransposition of a fully processed PCBP-2 mRNA. This gene andPCBP-2 have paralogues (PCBP3 and PCBP4) which are thought to havearisen as a result of duplication events of entire genes. Theprotein encoded by this gene appears to be multifunctional. Italong with PCBP-2 and hnRNPK corresponds to the major cellularpoly(rC)-binding protein. It contains three K-homologous (KH)domains which may be involved in RNA binding. This encoded proteintogether with PCBP-2 also functions as translational coactivatorsof poliovirus RNA via a sequence-specific interaction withstem-loop IV of the IRES and promote poliovirus RNA replication bybinding to its 5'-terminal cloverleaf structure. It has also beenimplicated in translational control of the 15-lipoxygenase mRNA,human Papillomavirus type 16 L2 mRNA, and hepatitis A virus RNA.The encoded protein is also suggested to play a part in formationof a sequence-specific alpha-globin mRNP complex which isassociated with alpha-globin mRNA stability.
Background Reference 1:
Cloke, B., et al. Endocrinology 151(8):3954-3964(2010)
Background Reference 2:
Wang, H., et al. Cancer Cell 18(1):52-62(2010)
Background Reference 3:
Zhang, T., et al. Mol. Cancer 9, 72 (2010) :
Background Reference 4:
Waggoner, S.A., et al. J. Biol. Chem. 284(14):9039-9049(2009)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

60-150

List Size:
400 ul

List Price:

$495.00

Datasheet
Shipping Info

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