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Antibody used in WB on Transfected 293T at 0.2-1 ug/ml.
Antibody used in WB on Transfected 293T at 0.2-1 ug/ml.

PCCA Antibody

CATALOG NUMBER: 25-114

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human PCCA.
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
PCCA antibody can be used for detection of PCCA by ELISA at 1:312500. PCCA antibody can be used for detection of PCCA by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Positive Control 1:
Tranfected 293T Cell Lysate
Predicted Molecular Weight:
75 kDa
Purification:
Antibody is purified by peptide affinity chromatography method.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Concentration:
batch dependent
Storage Conditions:
For short periods of storage (days) store at 4°C. For longer periods of storage, store PCCA antibody at -20°C. As with any antibody avoid repeat freeze-thaw cycles.
Ncbi Official Symbol:
PCCA
Additional Names:
PCCA,
Protein Accession Number:
NP_000273
Protein Gi Number:
65506442
Ncbi Gene Id Number:
5095
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
PCCA is the alpha subunit of the heterodimeric mitochondrial enzyme Propionyl-CoA carboxylase. PCCA is the biotin-binding region of this enzyme. Mutations in either PCCA or PCCB (encoding the beta subunit) lead to an enzyme deficiency resulting in propionic acidemia.The protein encoded by this gene is the alpha subunit of the heterodimeric mitochondrial enzyme Propionyl-CoA carboxylase. PCCA encodes the biotin-binding region of this enzyme. Mutations in either PCCA or PCCB (encoding the beta subunit) lead to an enzyme deficiency resulting in propionic acidemia. Two transcript variants encoding different isoforms have been found for this gene.
Background Reference 1:
Ugarte, M., (er) Am. J. Hum. Genet. 81 (6) (2007) In press.

FOR RESEARCH USE ONLY

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Disclaimer:
This product is for research use only.

CATALOG NUMBER:

25-114

List Size:
100 ul

List Price:

$519.00

Datasheet
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