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Antibody staining PCCA in human kidney tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections).
Antibody staining PCCA in human heart tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections).
Antibody staining PCCA in human kidney tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections).
Antibody staining PCCA in human heart tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections).
Western Blot at 1:2000 dilution + human liver lysate Lysates/proteins at 20 ug per lane.
Western Blot at 1:2000 dilution Lane 1: human liver lysate Lane 2: NIH/3T3 whole cell lysate Lysates/proteins at 20 ug per lane.
Western Blot at 1:2000 dilution Lane 1: Hela whole cell lysate Lane 2: rat cerebellum lysate Lysates/proteins at 20 ug per lane.
Antibody staining PCCA in human kidney tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections).

PCCA Antibody

CATALOG NUMBER: 56-351

Clonality:
Polyclonal
Tested Applications:
IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Rat
Immunogen:
This PCCA antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 362-390 amino acids from the Central region of human PCCA.
Conjugate:
Unconjugated
Tested Applications:
IHC-P, WB
Application Note:
For IHC-P starting dilution is: 1:25
For WB starting dilution is: 1:2000
Predicted Molecular Weight:
80 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
PCCA
Additional Names:
Propionyl-CoA carboxylase alpha chain, mitochondrial, PCCase subunit alpha, Propanoyl-CoA:carbon dioxide ligase subunit alpha, PCCA
Protein Accession Number:
P05165
Protein Gi Number:
308153661
Ncbi Gene Id Number:
5095
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The protein encoded by this gene is the alpha subunit ofthe heterodimeric mitochondrial enzyme Propionyl-CoA carboxylase.PCCA encodes the biotin-binding region of this enzyme. Mutations ineither PCCA or PCCB (encoding the beta subunit) lead to an enzymedeficiency resulting in propionic acidemia. Multiple transcriptvariants encoding different isoforms have been found for this gene.
Background Reference 1:
Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010)
Background Reference 2:
Huang, C.S., et al. Nature 466(7309):1001-1005(2010)
Background Reference 3:
Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010) :
Background Reference 4:
Talmud, P.J., et al. Am. J. Hum. Genet. 85(5):628-642(2009)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

56-351

List Size:
400 ul

List Price:

$495.00

Datasheet
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