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Western blot analysis of PCDH12 in K562 cell lysate with PCDH12 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.
Immunohistochemistry of PCDH12 in rat lung tissue with PCDH12 antibody at 5 μg/mL.
Western blot analysis of PCDH12 in K562 cell lysate with PCDH12 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.
Trial Size

PCDH12 Antibody

CATALOG NUMBER: 5071

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
PCDH12 antibody was raised against a 17 amino acid synthetic peptide near the amino terminus of human PCDH12.
The immunogen is located within amino acids 30 - 80 of PCDH12.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC-P, WB
Application Note:
PCDH12 antibody can be used for detection of PCDH12 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL.
Antibody validated: Western Blot in human samples and Immunohistochemistry in rat samples. All other applications and species not yet tested.
Specificity:
This antibody is predicted to not cross-react with PCDH18.
Positive Control 1:
Cat. No. 1204 - K562 Cell Lysate
Predicted Molecular Weight:
Predicted: 128 kDa
Observed: 125 kDa
Purification:
PCDH12 Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
Physical State:
Liquid
Buffer:
PCDH12 Antibody is supplied in PBS containing ≤0.09% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
PCDH12 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
PCDH12
Additional Names:
PCDH12 Antibody: VECAD2, VE-cadherin-2, Protocadherin-12, Vascular cadherin-2, VE-cad-2
Protein Accession Number:
NP_057664
Protein Gi Number:
7706113
Ncbi Gene Id Number:
51294
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
PCDH12 Antibody: Protocadherins comprise the largest group within the cadherin family of calcium-dependent cell-cell adhesion molecules. Protocadherin 12 (PCDH12) was initially identified through PCR screening of mouse heart microvascular endothelial cell RNA; further experiments revealed its mRNA to be strongly expressed in highly vascularized organs such as lung and kidney, in addition to glycogen-rich trophoblasts in the placenta. PCDH12-null mice are viable and fertile, but show reduced placental and embryonic sizes when compared to wild-type mice. Further studies showed significant expression changes in 2, 289 genes, including those involved in tissue morphogenesis, angiogenesis, cell-matrix adhesion and migration, immune response and chromatin remodeling.
Background Reference 1:
Frank M and Kemler R. Protocadherins. Curr. Opin. Cell Biol.2002; 14:557-62.
Background Reference 2:
Telo P, Breviaro F, Huber P, et al. Identification of a novel cadherin (vascular endothelial cadherin-2) located at intercellular junctions in endothelial cells. J. Biol. Chem.1998; 28:17565-72.
Background Reference 3:
Rampon C, Prandini MH, Bouillot S, et al. Protocadherin 12 (VE-cadherin 2) is expressed in endothelial, trophoblast, and mesangial cells. Exp. Cell Res.2005; 302:48-60.
Background Reference 4:
Rampon C, Bouillot S, Climescu-Haulica A, et al. Protocadherin 12 deficiency alters morphogenesis and transcriptional profile of the placenta. Physiol. Genomics2008; 34:193-204.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

5071

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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