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Western blot analysis of PCDH18 in HepG2 cell lysate with PCDH18 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.
Immunohistochemistry of PCDH18 in mouse brain tissue with PCDH18 antibody at 2.5 μg/mL.
Immunofluorescence of PCDH18 in Mouse Brain cells with PCDH18 antibody at 20 μg/mL.
Western blot analysis of PCDH18 in HepG2 cell lysate with PCDH18 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.
Trial Size

PCDH18 Antibody

CATALOG NUMBER: 5081

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Homology:
Predicted species reactivity based on immunogen sequence: Bovine: (95%)
Immunogen:
PCDH18 antibody was raised against a 17 amino acid synthetic peptide near the amino terminus of human PCDH18.
The immunogen is located within amino acids 50 - 100 of PCDH18.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, IHC-P, WB
Application Note:
PCDH18 antibody can be used for detection of PCDH18 by Western blot at 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.
Specificity:
At least three isoforms of PCDH18 are known to exist. This antibody is predicted to not cross-react with PCDH12.
Positive Control 1:
Cat. No. 1211 - HepG2 Cell Lysate
Purification:
PCDH18 Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
Physical State:
Liquid
Buffer:
PCDH18 Antibody is supplied in PBS containing ≤0.09% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
PCDH18 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
PCDH18
Additional Names:
PCDH18 Antibody: PCDH68L, KIAA1562, Protocadherin-18
Protein Accession Number:
NP_061908
Protein Gi Number:
14589929
Ncbi Gene Id Number:
54510
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
PCDH18 Antibody: Protocadherins comprise the largest group within the cadherin family of calcium-dependent cell-cell adhesion molecules. Protocadherin 18 (PCDH18) was initially identified through screening of human and mouse ESTs using conserved cytoplasmic domain motifs. Tissue screening revealed PCDH18 broad expression in tissues such as brain, liver, heart, kidney, lung, and trachea. PCDH18 expression is also temporally and spatially regulated in the mouse embryonic brain and interacts with Disabled-1, an intracellular adapter protein involved in neuronal migration and cell positioning during mammalian brain development. PCDH18 was also found to be expressed in the developing zebrafish neural tube and central nervous system, lending support to the hypothesis that PCDH18 may play a role during brain development.
Background Reference 1:
Frank M and Kemler R. Protocadherins. Curr. Opin. Cell Biol.2002; 14:557-62.
Background Reference 2:
Wolverton T and Lalande M. Identification and characterization of three members of a novel subclass of protocadherins. Genomics2001; 76:66-72.
Background Reference 3:
Homayouni R, Rice DS, and Curran T. Disabled-1 interacts with a novel developmentally regulated protocadherin. Biochem. Biophys. Res. Commun.2001; 289:539-47.
Background Reference 4:
Kubota F, Murakami T, Tajika Y, et al. Expression of protocadherin 18 in the CNS and pharyngeal arches of zebrafish embryos. Int. J. Dev. Biol.2008; 52:397-405.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

5081

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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