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Western blot analysis in MCF-7 cell line lysates (35ug/lane).
PCDH20 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human pancreas tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
Confocal immunofluorescent analysis of PCDH20 Antibody with MCF-7 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).
Flow cytometric analysis of MCF-7 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western blot analysis in MCF-7 cell line lysates (35ug/lane).

PCDH20 Antibody

CATALOG NUMBER: 55-965

Clonality:
Polyclonal
Tested Applications:
Flow, IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This PCDH20 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 445-472 amino acids from the Central region of human PCDH20.
Conjugate:
Unconjugated
Tested Applications:
Flow, IF, IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:10~50
For IF starting dilution is: 1:10~50
For FACS starting dilution is: 1:10~50
Predicted Molecular Weight:
105 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
PCDH20
Additional Names:
Protocadherin-20, Protocadherin-13, PCDH20, PCDH13
Protein Accession Number:
Q8N6Y1
Protein Gi Number:
544584765
Ncbi Gene Id Number:
64881
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This gene belongs to the protocadherin gene family, asubfamily of the cadherin superfamily. This gene encodes a proteinwhich contains 6 extracellular cadherin domains, a transmembranedomain and a cytoplasmic tail differing from those of the classicalcadherins. Although its specific function is undetermined, thecadherin-related neuronal receptor is thought to play a role in theestablishment and function of specific cell-cell connections in thebrain.
Background Reference 1:
Suzuki, S.T. Exp. Cell Res. 261(1):13-18(2000)
Background Reference 2:
Nollet, F., et al. J. Mol. Biol. 299(3):551-572(2000)
Background Reference 3:
Yagi, T., et al. Genes Dev. 14(10):1169-1180(2000)
Background Reference 4:
Wu, Q., et al. Proc. Natl. Acad. Sci. U.S.A. 97(7):3124-3129(2000)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

55-965

List Size:
400 ul

List Price:

$495.00

Datasheet
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