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Western blot analysis in Ramos cell line lysates (35ug/lane).
PCDHGA3 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
Flow cytometric analysis of Ramos cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.
Western blot analysis in Ramos cell line lysates (35ug/lane).

PCDHGA3 Antibody

CATALOG NUMBER: 57-156

Clonality:
Polyclonal
Tested Applications:
Flow, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This PCDHGA3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 284-311 amino acids from the Central region of human PCDHGA3.
Conjugate:
Unconjugated
Tested Applications:
Flow, IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:10~50
For FACS starting dilution is: 1:10~50
Predicted Molecular Weight:
101 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
PCDHGA3
Additional Names:
Protocadherin gamma-A3, PCDH-gamma-A3, PCDHGA3
Protein Accession Number:
Q9Y5H0
Protein Gi Number:
296439266
Ncbi Gene Id Number:
56112
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This gene is a member of the protocadherin gamma genecluster, one of three related clusters tandemly linked onchromosome five. These gene clusters have an immunoglobulin-likeorganization, suggesting that a novel mechanism may be involved intheir regulation and expression. The gamma gene cluster includes 22genes divided into 3 subfamilies. Subfamily A contains 12 genes,subfamily B contains 7 genes and 2 pseudogenes, and the moredistantly related subfamily C contains 3 genes. The tandem array of22 large, variable region exons are followed by a constant region,containing 3 exons shared by all genes in the cluster. Eachvariable region exon encodes the extracellular region, whichincludes 6 cadherin ectodomains and a transmembrane region. Theconstant region exons encode the common cytoplasmic region. Theseneural cadherin-like cell adhesion proteins most likely play acritical role in the establishment and function of specificcell-cell connections in the brain. Alternative splicing has beendescribed for the gamma cluster genes.
Background Reference 1:
Rose, J. Phd, et al. Mol. Med. (2010) In press :
Background Reference 2:
Wu, Q., et al. Genome Res. 11(3):389-404(2001)
Background Reference 3:
Nollet, F., et al. J. Mol. Biol. 299(3):551-572(2000)
Background Reference 4:
Yagi, T., et al. Genes Dev. 14(10):1169-1180(2000)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

57-156

List Size:
400 ul

List Price:

$495.00

Datasheet
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