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Western blot analysis of PCM-1 antibody (C-eterm) in K562 cell line lysates (35ug/lane)
Formalin-fixed and paraffin-embedded human lymph reacted with PCM-1 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Flow cytometric analysis of K562 cells using PCM-1 Antibody (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western blot analysis of PCM-1 antibody (C-eterm) in K562 cell line lysates (35ug/lane)

PCM-1 Antibody

CATALOG NUMBER: 62-943

Clonality:
Polyclonal
Tested Applications:
Flow, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This PCM-1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1816-1845 amino acids from the C-terminal region of human PCM-1.
Conjugate:
Unconjugated
Tested Applications:
Flow, IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:50~100
For FACS starting dilution is: 1:10~50
Predicted Molecular Weight:
229 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
PCM1
Additional Names:
Pericentriolar material 1 protein, PCM-1, hPCM-1, PCM1
Protein Accession Number:
Q15154
Protein Gi Number:
296439495
Ncbi Gene Id Number:
5108
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
PCM-1 is required for centrosome assembly and function. This protein is essential for the correct localization of several centrosomal proteins including CEP250, CETN3, PCNT and NEK2. The protein is required to anchor microtubules to the centrosome.
Background Reference 1:
Balczon R., Bao L.J. Cell Biol. 124:783-793(1994)
Background Reference 2:
Corvi R., Berger N.Oncogene 19:4236-4242(2000)
Background Reference 3:
Dammermann A., Merdes A.J. Cell Biol. 159:255-266(2002)
Background Reference 4:
Reiter A., Walz C.Cancer Res. 65:2662-2667(2005)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

62-943

List Size:
400 ul

List Price:

$495.00

Datasheet
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