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Western blot analysis of extracts of various cell lines, using PCMT1 antibody (22-403).<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 30s.
Immunofluorescence analysis of HeLa cells using PCMT1 antibody (22-403). Blue: DAPI for nuclear staining.
Immunoprecipitation analysis of 200ug extracts of HepG2 cells using 1ug PCMT1 antibody (22-403). Western blot was performed from the immunoprecipitate using PCMT1 antibody (22-403) at a dilition of 1:1000.
Western blot analysis of extracts of various cell lines, using PCMT1 antibody (22-403).
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 30s.

PCMT1 Antibody

CATALOG NUMBER: 22-403

Clonality:
Polyclonal
Tested Applications:
IF, IP, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 62-286 of human PCMT1 (NP_001238978.1).
Conjugate:
Unconjugated
Tested Applications:
IF, IP, WB
Application Note:
WB: 1:500 - 1:2000
IF: 1:50 - 1:100
IP: 1:50 - 1:200
Positive Control 1:
MCF7
Positive Control 2:
HepG2
Positive Control 3:
293T
Positive Control 4:
A-431
Positive Control 5:
Mouse liver
Positive Control 6:
Mouse brain
Predicted Molecular Weight:
Observed: 25kDa
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
PCMT1
Additional Names:
PCMT1, PIMT
Ncbi Gene Id Number:
5110
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This gene encodes a member of the type II class of protein carboxyl methyltransferase enzymes. The encoded enzyme plays a role in protein repair by recognizing and converting D-aspartyl and L-isoaspartyl residues resulting from spontaneous deamidation back to the normal L-aspartyl form. The encoded protein may play a protective role in the pathogenesis of Alzheimer's disease, and single nucleotide polymorphisms in this gene have been associated with spina bifida and premature ovarian failure. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

22-403

List Size:
100 uL

List Price:

$436.00

Datasheet
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