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Antibody used in IHC on Human Spleen at 4.0-8.0 ug/ml.
Antibody used in IHC on Human brain stem at: 1:50.
Antibody used in WB on Human HepG2 at 1.25 ug/ml.
Antibody used in WB on Human NT-2 at 2 ug/ml.
Antibody used in IHC on Human NT-2 at 1ug/50ul.
Antibody used in IHC on Human Spleen at 4.0-8.0 ug/ml.

PCNA Antibody

CATALOG NUMBER: 31-033

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC, WB
Host Species:
Rabbit
Species Reactivity:
Dog, Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Dog, Human, Mouse, Rat
Immunogen:
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human PCNA.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC, WB
Application Note:
PCNA antibody can be used for detection of PCNA by ELISA at 1:62500. PCNA antibody can be used for detection of PCNA by western blot at 1.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Positive Control 1:
Cat. No. 1211 - HepG2 Cell Lysate
Predicted Molecular Weight:
29 kDa, 29 kDa
Purification:
Antibody is purified by protein A chromatography method.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Concentration:
batch dependent
Storage Conditions:
For short periods of storage (days) store at 4°C. For longer periods of storage, store PCNA antibody at -20°C. As with any antibody avoid repeat freeze-thaw cycles.
Ncbi Official Symbol:
PCNA
Additional Names:
PCNA, MGC8367,
Protein Accession Number:
NP_002583
Protein Gi Number:
4505641
Ncbi Gene Id Number:
5111
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
PCNA is an auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand.The protein encoded by this gene is found in the nucleus and is a cofactor of DNA polymerase delta. The encoded protein acts as a homotrimer and helps increase the processivity of leading strand synthesis during DNA replication. In response to DNA damage, this protein is ubiquitinated and is involved in the RAD6-dependent DNA repair pathway. Two transcript variants encoding the same protein have been found for this gene. Pseudogenes of this gene have been described on chromosome 4 and on the X chromosome.
Background Reference 1:
Senga, T., (2006) J. Biol. Chem. 281 (10), 6246-6252.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
This product is for research use only.

CATALOG NUMBER:

31-033

List Size:
100 ul

List Price:

$473.00

Datasheet
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