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43-158 (0.01ug/ml) staining of HeLa lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
43-158 (0.01ug/ml) staining of NIH3T3 (A), Mouse Testis (B) and Rat Testis (C) lysates (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
43-158 (0.03ug/ml) staining of Pig Spleen lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
43-158 (0.01ug/ml) staining of HeLa lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

PCNA Antibody

CATALOG NUMBER: 43-158

Clonality:
Polyclonal
Tested Applications:
EIA, ELISA, WB
Host Species:
Goat
Species Reactivity:
Human, Mouse, Pig, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human, Mouse, Pig, Rat
Homology:
Expected Species Reactivity based on sequence homology: Dog, Cow
Immunogen:
The immunogen for this antibody is: C-NGNIKLSQTSNVD
Conjugate:
Unconjugated
Tested Applications:
EIA, ELISA, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 30kDa band observed in lysates of cell line HeLa (calculated MW of 28.8kDa according to Human NP_002583.1). Approx.33kDa band observed in lysates of cell line NIH3T3 and in Mouse and Rat Testis and Pig Spleen lysates (calculated MW of 28.8kDa according to Mouse NP_035175.1, Rat NP_071776.1 and Pig NP_001278854.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Gilljam et al, , J Cell Biol. 2009 Sep 7;186(5):645-54.). Recommended concentration: 0.01-0.03ug/ml.Enzyme immunoassay:This antibody was reported to work in sandwich-type ELISA both as a capturer and as a reporter in combination with EB11651.

Additional validation: This antibody has been successfully used in the following paper: Sikorski et al. (2018) PMID: 30377371.

Specificity:
Reported variants represent identical protein: NP_872590.1, NP_002583.1
Predicted Molecular Weight:
Approx 30kDa
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
PCNA
Additional Names:
PCNA, proliferating cell nuclear antigen, MGC8367, DNA polymerase delta auxiliary protein, OTTHUMP00000030189, OTTHUMP00000030190, cyclin
Protein Accession Number:
NP_002583.1
Protein Gi Number:
4505641
Ncbi Gene Id Number:
5111
Background Reference 1:
Jones MJ, Colnaghi L, Huang TT.Dysregulation of DNA polymerase κ recruitment to replication forks results in genomic instability.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

43-158

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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