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Formalin-fixed, paraffin-embedded human tonsil stained with anti-PCNA antibody (SPM350)
Western blot testing of A) human HeLa and B) mouse NIH3T3 cell lysates with anti-PCNA antibody (clone SPM350). Predicted molecular weight ~29kDa, routinely observed at 29~36kDa.
Formalin-fixed, paraffin-embedded human tonsil stained with anti-PCNA antibody (SPM350)

PCNA Antibody [SPM350]

CATALOG NUMBER: 34-150

Clonality:
Monoclonal
Tested Applications:
Flow, IF, IHC, WB
Host Species:
Mouse
Species Reactivity:
Chicken, Drosophila, Human, Monkey, Mouse, Pig, Rat, Yeast, Zebrafish
Conjugate:
Unconjugated
Specifications
Host Species:
Mouse
Species Reactivity:
Chicken, Drosophila, Human, Monkey, Mouse, Pig, Rat, Yeast, Zebrafish
Immunogen:
Rat PCNA protein was used as the immunogen for this anti-PCNA antibody.
Conjugate:
Unconjugated
Tested Applications:
Flow, IF, IHC, WB
Application Note:
Flow Cytometry: 0.5-1 ug/million cells in 0.1ml
Immunofluorescence: 0.5-1 ug/ml
Western blot: 0.5-1 ug/ml
Immunohistochemistry (FFPE): 0.25-0.5 ug/ml for 30 min at RT (1)
Prediluted format: incubate for 30 min at RT (2)
The optimal dilution of the anti-PCNA antibody for each application should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Purification:
Protein G affinity chromatography
Clonality:
Monoclonal
Isotype:
IgG2a, kappa
physical-state:
Liquid
Buffer:
PBS with 0.1 mg/ml BSA and 0.05% sodium azide
Concentration:
0.2 mg/mL
Storage Conditions:
Aliquot and Store at 2-8°C. Avoid freez-thaw cycles.
Ncbi Official Symbol:
PCNA
Additional Names:
Proliferating cell nuclear antigen, PCNA, Cyclin, PCNA
Ncbi Gene Id Number:
5111
User Note:
Optimal dilutions for each application to be determined by the researcher
Background:
Recognizes a non-histone protein of 36kDa, which is identified as proliferating cell nuclear antigen (PCNA). It is also known as cyclin or polymerase delta auxiliary protein. Elevated expression of PCNA/cyclin has been shown in the nucleus during late G1 phase immediately before the onset of DNA synthesis, becoming maximal during S-phase and declining during G2 and M phases.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

34-150

List Size:
100 ug

List Price:

$626.00

Datasheet
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