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43-182 (0.3ug/ml) staining of Human Skin lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
43-182 (0.3ug/ml) staining of Human Skin lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

PCOLCE Antibody

CATALOG NUMBER: 43-182

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Homology:
Expected Species Reactivity based on sequence homology: Dog
Immunogen:
The immunogen for this antibody is: QILTNLSKRKCPSQP
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 55kDa band observed in Human Skin and Umbilical Cord lysates (calculated MW of 48.0kDa according to NP_002584.2). Recommended concentration: 0.3-1ug/ml.
Predicted Molecular Weight:
Approx 55kDa
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
PCOLCE
Additional Names:
PCOLCE, procollagen C-endopeptidase enhancer, PCPE, PCPE-1, PCPE1, OTTHUMP00000210409, procollagen C-endopeptidase enhancer 1, procollagen C-proteinase enhancer 1, procollagen COOH-terminal proteinase enhancer 1, procollagen, type 1, COOH-terminal proteinase enhancer, type 1 procollagen C-proteinase enhancer protein, type I procollagen COOH-terminal proteinase enhancer
Protein Accession Number:
NP_002584.2
Protein Gi Number:
157653329
Ncbi Gene Id Number:
5118
Background Reference 1:
Vadon-Le Goff S, Kronenberg D, Bourhis JM, Bijakowski C, Raynal N, Ruggiero F, Farndale RW, Stöcker W, Hulmes DJ, Moali C.Procollagen C-proteinase enhancer stimulates procollagen processing by binding to the C-propeptide region only.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

43-182

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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