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Western Blot at 1:1000 dilution + human cerebellum lysate Lysates/proteins at 20 ug per lane.
Formalin-fixed and paraffin-embedded human brain tissue reacted with hPCPTP1-Y249, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Western Blot at 1:1000 dilution + human cerebellum lysate Lysates/proteins at 20 ug per lane.

PCPTP1 Antibody

CATALOG NUMBER: 63-457

Clonality:
Polyclonal
Tested Applications:
IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This PCPTP1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 234-265 amino acids from the N-terminal region of human PCPTP1.
Conjugate:
Unconjugated
Tested Applications:
IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:10~50
Predicted Molecular Weight:
74 kDa
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
PTPRR
Additional Names:
Receptor-type tyrosine-protein phosphatase R, R-PTP-R, Ch-1PTPase, NC-PTPCOM1, Protein-tyrosine phosphatase PCPTP1, PTPRR, ECPTP, PTPRQ
Protein Accession Number:
Q15256
Protein Gi Number:
134039192
Ncbi Gene Id Number:
5801
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
PCPTP1 is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. This PTP possesses an extracellular region, a single transmembrane region, and a single intracellular catalytic domains, and thus represents a receptor-type PTP. The similar gene for this protein predominately expressed in mouse brain was found to associate with, and thus regulate the activity and cellular localization of MAP kinases. The rat counterpart of the gene for this protein was reported to be regulated by the nerve growth factor, which suggested a function in neuronal growth and differentiation.
Background Reference 1:
Blanco-Aparicio, C., et al., J. Cell Biol. 147(6):1129-1136 (1999).
Background Reference 2:
Ogata, M., et al., J. Biol. Chem. 270(5):2337-2343 (1995).
Background Reference 3:
Shiozuka, K., et al., Gene 162(2):279-284 (1995).
Background Reference 4:
Sharma, E., et al., J. Biol. Chem. 270(1):49-53 (1995).

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

63-457

List Size:
400 ul

List Price:

$495.00

Datasheet
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