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HEK293 lysate (10ug protein in RIPA buffer) overexpressing Human PCSK9 with C-terminal MYC tag probed with 42-463 (0.5ug/ml) in Lane A and probed with anti-MYC Tag (1/1000) in lane C. Mock-transfected HEK293 probed with 42-463 (1mg/ml) in Lane B. Primar
HEK293 lysate (10ug protein in RIPA buffer) overexpressing Human PCSK9 with C-terminal MYC tag probed with 42-463 (0.5ug/ml) in Lane A and probed with anti-MYC Tag (1/1000) in lane C. Mock-transfected HEK293 probed with 42-463 (1mg/ml) in Lane B. Primar

PCSK9 Antibody

CATALOG NUMBER: 42-463

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Immunogen:
The immunogen for this antibody is: C-PRYRADEYQPPD
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:In transfected HEK293 transiently expressing full-length Human PCSK9 (myc and DYKDDDDK tagged), a doublet of approx. 80-100kDa was observed. No bands were observed in mock-transfected HEK293 and the same bands were observed using anti-myc tag antibody. Recommended concentration, 0.1-0.5ug/ml.Purchase the +ve control! The transfected lysate used to QC this product is available for sale. Please email sales@everestbiotech.com to request datasheet, pricing and delivery information.
Positive Control 1:
Transfected HEK293
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
PCSK9
Additional Names:
PCSK9, proprotein convertase subtilisin/kexin type 9 , FH3, HCHOLA3, NARC-1, NARC1 , hypercholesterolemia, autosomal dominant 3, neural apoptosis regulated convertase 1
Protein Accession Number:
NP_777596.2
Protein Gi Number:
31317307
Ncbi Gene Id Number:
255738

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

42-463

List Size:
0.1 mg

List Price:

$495.00

Datasheet
Shipping Info

$65 Standard Overnight flat rate for as many products shipped within the United States.

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