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46-147 (4ug/ml) staining of paraffin embedded Human Kidney. Steamed antigen retrieval with Tris/EDTA buffer pH 9, HRP-staining. <strong>This data is from a previous batch, not on sale.</strong>
46-147 (0.3ug/ml) staining of Human Adipose lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
46-147 (4ug/ml) staining of paraffin embedded Human Kidney. Steamed antigen retrieval with Tris/EDTA buffer pH 9, HRP-staining. This data is from a previous batch, not on sale.

PCSK9 Antibody

CATALOG NUMBER: 46-147

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Immunogen:
The immunogen for this antibody is: CRSRHLAQASQELQ
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Approx 75kDa band observed in Human Adipose lysates (calculated MW of 74.3kDa according to NP_777596.2). Recommended concentration: 0.3-1ug/ml. Primary incubation was 1 hour. Constructs and lysates were as described in Sun et al Hum Mol Genet. 2005 May 1;14(9):1161-9, PMID: 15772090. A customer found positive results in WB on Human Liver.
Positive Control 1:
McA Cell Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
PCSK9
Additional Names:
PCSK9, FH3, NARC1, HCHOLA3, proprotein convertase subtilisin/kexin type 9, neural apoptosis regulated convertase 1, hypercholesterolemia, autosomal dominant 3, proprotein convertase subtilisin/kexin type 9, LDLCQ1, NARC-1
Protein Accession Number:
NP_777596.2
Protein Gi Number:
31317307
Ncbi Gene Id Number:
255738
Background Reference 1:
Cohen J, Pertsemlidis A, Kotowski IK, Graham R, Garcia CK, Hobbs HH. Low LDL cholesterol in individuals of African descent resulting from frequent nonsense mutations in PCSK9. Nat Genet. 2005 Feb;37(2):161-165. Epub 2005 Jan 16.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

46-147

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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